Description

IL-1α (Interleukin-1α), a key member of the interleukin-1 (IL-1) family, shares a similar β-trefoil topology with other IL-1 family members such as IL-1β, despite low sequence homology. Like IL-1β, it exerts its biological functions by binding to the interleukin-1 receptor (IL-1R). IL-1α is a pleiotropic cytokine that plays a central role in inflammation, immune responses, and cell proliferation.In in vitro cell culture, IL-1α stimulates monocytes/macrophages and dendritic cells to secrete other cytokines, thereby mimicking an inflammatory environment. It also promotes the proliferation of fibroblasts and endothelial cells, making it a useful tool in wound healing and angiogenesis research.

Specifications

Product Details
Cat. No.	Y01921H
Purity	≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤100 EU/mg by the LAL method.
Bioactivity	Measured by its ability to induce NF-kB reporter activity by the reporter gene assay. The ED50 for this effect is ≤0.05ng/mL.
Accession	P01583
Species	Human
Expression System	E. coli
Mol. Weight	18.0kDa
Expression Region	Ser113-Ala271
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PB, 5% Trehalose and 0.01% Tween 80, pH7.4.
Size	50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Involved in inflammation-driven hematopoietic stress, affecting myeloid cell activation.

Immunology

Classical pro-inflammatory cytokine that activates innate immunity and amplifies inflammatory cascades.

Cell Biology

Regulates inflammatory responses, stress signaling, and intercellular communication.

Data Figures

Human IL-1A (Cat. No. Y01921H) induces NF-kB reporter activity by the reporter gene assay. The ED50 for this effect is ≤0.05 ng/mL. The activity of Human IL-1A (Cat. No. Y01921H) was higher than the other competing product.

Figure 1: Human IL-1A (Cat. No. Y01921H) induces NF-kB reporter activity by the reporter gene assay. The ED50 for this effect is ≤0.05 ng/mL. The activity of Human IL-1A (Cat. No. Y01921H) was higher than the other competing product.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human IL-1A