Description

IL-18 (Interleukin-18) is a pleiotropic pro-inflammatory cytokine belonging to the IL-1 superfamily. It signals through a heterodimeric receptor composed of IL-18Rα (binding chain) and IL-18Rβ (signaling chain), leading to activation of NF-κB and MAPK pathways. IL-18 exerts immunomodulatory functions by promoting Th1 immune responses and, in synergy with IL-12, induces IFN-γ production in T cells and NK cells, thereby enhancing cell-mediated immunity (e.g., in anti-tumor or anti-infection responses). Additionally, it activates NK cells, boosting their cytotoxicity and cytokine secretion. Under specific conditions—such as in combination with IL-23—IL-18 can also promote Th17 differentiation and has been implicated in models of autoimmune diseases.

Specifications

Product Details
Cat. No.	Y02821H
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is ≤2.0 ng/mL.
Accession	Q14116
Alternative Names	Interleukin-18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma;GIF; IL-18; IL-1g; IL1F4; MG/VAIL C12320
Species	Human
Expression System	CHO
Mol. Weight	18.2 kDa
Expression Region	Tyr37-Asp193
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at 2°C to 8°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Supports maturation of NK and T cell functions.

Immunology

Enhances IFN-γ production, amplifying inflammatory responses.

Cell Biology

Regulates inflammation and cell activation signaling.

Data Figures

Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is ≤2.0 ng/mL.

Figure 1: Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is ≤2.0 ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human IL-18