Description

IL-15 (Interleukin-15) is a pleiotropic cytokine belonging to the common gamma-chain (γc) cytokine family. While structurally similar to IL-2, it exhibits distinct functional properties. It is primarily produced by antigen-presenting cells (such as dendritic cells and macrophages), epithelial cells, and fibroblasts, and acts through activation of the JAK-STAT signaling pathway, predominantly via JAK1/3 and STAT3/5. IL-15 is essential for the survival of memory CD8⁺ T cells and natural killer (NK) cells. In serum-free culture systems, it can substitute for serum by providing crucial survival signals, thereby promoting the persistence and proliferation of T and NK cells while enhancing NK cell cytotoxicity. As a key tool in tumor immunology, infectious immunity, and cell therapy research, IL-15 plays an especially indispensable role in experiments requiring long-term maintenance of immune cell activity.

Specifications

Product Details
Cat. No.	Y02701
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is ≤0.1 ng/mL，corresponding to a specific bioactivity of ≥ 2 x 107 units/mg.
Accession	P40933
Alternative Names	Interleukin-15; IL-15; IL15
Species	Human
Expression System	CHO
Mol. Weight	12.7 kDa
Expression Region	Asn49-Ser162
Tag Info	Tag free
Form	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at 2°C to 8°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Promotes NK and memory T cell generation.

Immunology

Maintains long-term survival of NK and CD8+ T cells.

Cell Biology

Regulates metabolism, survival, and functional maintenance.

Data Figures

Figure 1: Recombinant Human IL-15 (Cat. No. Y02701) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y02701 SDS-PAGE.

Figure 2: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is ≤0.5 ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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