Description

IGF-2 (Insulin-like Growth Factor 2) is an important single-chain polypeptide growth factor composed of 67 amino acids with a molecular weight of approximately 7.5 kDa. It shares structural similarity with insulin and IGF-1 and belongs to the insulin-like growth factor family. In vivo, IGF-2 often binds to IGF-binding proteins (IGFBPs), which regulate its stability and activity. IGF-2 stimulates DNA synthesis and mitosis in various cell types, such as fibroblasts, muscle cells, and neural cells. It is commonly used in stem cell culture—including embryonic stem cells and mesenchymal stem cells—to sustain proliferative capacity. Under specific conditions and in combination with other factors, it can induce differentiation into muscle, cartilage, or neural cells. Additionally, IGF-2 plays regulatory roles in embryonic development and tumor cell growth.

Specifications

Product Details
Cat. No.	Y12441H
Purity	≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is ≤50 ng/mL.
Accession	P01344.1
Species	Human
Expression System	HEK293
Mol. Weight	7.5kDa
Expression Region	Ala25-Glu91
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm filtered solution containing TFA, ACN, 5% Trehalose.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in 4 mM HCl.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Modulates fetal hematopoiesis and progenitor expansion.

Stem Cell Research

Maintains stem cell proliferation and supports organoid growth.

Cell Biology

Controls cell proliferation, survival, and growth factor signaling.

Data Figures

Figure 1: Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is ≤50 ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human IGF-2