Background

Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a pivotal glycosylated cytokine functioning extracellularly as a homodimer. Its core function is to stimulate the proliferation and differentiation of hematopoietic stem/progenitor cells into granulocytes and monocytes (which mature into macrophages and dendritic cells).

Leveraging this, GM-CSF finds extensive application in cell and gene therapy. In cell therapy, it is a critical factor for the in vitro expansion of immune cells (e.g., dendritic cells for DC vaccines) and acts as an immune modulator in vivo to activate DCs and T cells, enhancing anti-tumor immunity.

In gene therapy, its encoding gene (located in the chromosome 5q31 region) serves as a potential target, or therapeutic GM-CSF can be delivered via vectors for localized and sustained expression.

Recombinantly produced GM-CSF protein provides an essential tool for these applications, with its potential dual immunomodulatory effects requiring careful evaluation.

Specifications

Product Details
Parameter	RUO Grade	GMP Grade
Catalog Number	TL-105-0010	GMP-TL105-0200
Synonyms	Interferon-gamma, Interferon-γ, Immune interferon	Interferon-gamma, Interferon-γ, Immune interferon
Accession	UniProt P01579	UniProt P01579
Expressed Region	Gln24–Gln166	Gln24–Gln166
Tag	C-terminal 6×His-tag	C-terminal 6×His-tag
Expression System	HEK293 cells	HEK293 cells
Molecular Weight	17.6 kDa	17.6 kDa
Purity	>90% (SDS-PAGE)	>90% (SDS-PAGE)
Endotoxin	<0.1 EU/µg	<0.1 EU/µg
Biological Activity	HT-29 cell proliferation inhibition assay, ≥ 1.0 × 10⁷ IU/mg	HT-29 cell proliferation inhibition assay, ≥ 1.0 × 10⁷ IU/mg
Formulation	Lyophilized from PBS (pH 7.4), 6–8% mannitol	Lyophilized from PBS (pH 7.4), 6–8% mannitol

Features

✔ High Purity: This product is purified using advanced chromatographic techniques, achieving a purity level of over 95%, ensuring reliable and reproducible results in experiments.
✔ High Biological Activity: Confirmed through multiple activity assays, this IFN-α protein effectively induces antiviral and antiproliferative effects in target cells, making it suitable for a range of research applications.
✔ Batch Consistency: Produced with rigorous quality control measures to ensure consistent quality between batches, reducing variability and enhancing experimental reliability.
✔ Animal-Free: Manufactured without animal-derived components, adhering to animal-free standards and broadening its applicability for various research purposes.

Applications

Virology

Study the antiviral mechanisms of IFN-α, providing insights into its role in controlling viral infections and developing antiviral therapies.

Immunology

Investigate the immunomodulatory effects of IFN-α on immune cell functions and interactions, contributing to a better understanding of immune responses and potential treatments for immune disorders.

Oncology

Explore the antiproliferative effects of IFN-α on cancer cells, offering valuable information for the development of cancer immunotherapies and treatment strategies.

Cell Culture

Utilize IFN-α to enhance cell culture systems, providing a more controlled environment for studying cellular responses and drug effects.

Stability & Storage

√ Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored inthe original sealed container under desiccant.
√ Reconstitution: Dissolve in sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4)maintaining final concentration ≥100 µg/mL to prevent adsorption.
√ Handling: Aliquot to avoid repeated freeze-thaw cycles.

Cautions

• Research Use Only: This product is not intended for diagnostic or therapeutic use.

• Handling: Follow recommended storage and handling instructions to maintain product stability.

• Support: Contact technical support if assistance is required.

Document

📄 GMP/RUO TL105 Data Sheet 📄 GMP/RUO TL105 SDS 📄 TL-105-0010 COA 📄 GMP-TL105-0200 COA

Recombinant Human IFN-γ Protein