Description

IFN-α2b (Interferon-α2b) is a cytokine produced by immune cells and belongs to the type I interferon family. It exerts antiviral, antiproliferative, and immunomodulatory effects primarily through activation of the JAK-STAT signaling pathway, and is widely used in the treatment of viral infections and tumors. Its antiviral activity is mediated by inducing the production of antiviral proteins that inhibit viral replication, making it useful for preventing or controlling viral contamination in cell culture. The antiproliferative function involves regulation of the cell cycle—such as arrest in the G1 phase—thereby inhibiting the growth of certain tumor cells or rapidly proliferating cell types, which supports research on cell cycle mechanisms and cancer therapy. Additionally, IFN-α2b enhances the expression of MHC class I molecules, promotes antigen presentation, and activates immune effector cells including NK cells and T cells, rendering it applicable in immunology-related studies.

Specifications

Product Details
Cat. No.	Y06301
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a VSV inhibition assay with wish cells. The ED50 this effect is ≤100 pg/mL, corresponding to a specific bioactivity is ≥2 ×108 units/mg.
Accession	P01563
Alternative Names	Interferon Alpha-2; IFN-Alpha-2; Interferon Alpha-A; LeIF A; IFNA2
Species	Human
Expression System	CHO
Mol. Weight	19.2 kDa
Expression Region	Cys24-Glu188
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Modulates hematopoiesis, can induce HSC quiescence.

Immunology

Antiviral cytokine activating innate immune responses.

Stem Cell Research

Used in vitro to model antiviral responses in stem cells.

Cell Biology

Regulates antiviral pathways, proliferation, and survival signaling.

Data Figures

Figure 1: Recombinant Human IFN-α2b (Cat. No. Y06301) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y06301 SDS-PAGE.

Measured in a VSV inhibition assay with Wish cells. The ED50 this effect is ≤100 pg/mL, corresponding to a specific bioactivity is ≥2 ×108 units/mg. The bioactivity of Y06301 was higher than the other competing product.

Figure 2: Measured in a VSV inhibition assay with Wish cells. The ED50 this effect is ≤100 pg/mL, corresponding to a specific bioactivity is ≥2 ×108 units/mg. The bioactivity of Y06301 was higher than the other competing product.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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Recombinant Human IFNα2b