{"product_id":"recombinant-human-holo-transferrin-holo","title":"Recombinant Human Holo-Transferrin (HOLO)","description":"\u003cstyle\u003e.biofargo-table-wrapper {overflow-x: auto;margin: 20px 0;-webkit-overflow-scrolling: touch;}.biofargo-spec-table {width: 100%;border-collapse: collapse;border: 1px solid #dee2e6;min-width: 600px;background: #fff;}.biofargo-spec-table th,.biofargo-spec-table td {border: 1px solid #dee2e6;padding: 12px;vertical-align: middle;white-space: normal;word-break: break-word;}@media (max-width: 600px) {.biofargo-table-wrapper {border: 1px solid #eee;border-radius: 8px;padding: 6px;background: #fff;}.biofargo-spec-table {min-width: 720px;}}\u003c\/style\u003e\n\u003cdiv style=\"font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1000px; margin: 0 auto; border: 1px solid #e1e8ed; padding: 30px; border-radius: 10px; background-color: #ffffff;\"\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 0; font-size: 22px;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fcfcfc; padding: 15px; border-radius: 6px; border: 1px solid #f0f0f0;\"\u003e\n\u003cp\u003eRecombinant Human Holo-Transferrin (HOLO) is an iron-saturated form of transferrin, a key glycoprotein responsible for iron transport and delivery in biological systems. Unlike apo-transferrin, holo-transferrin is fully loaded with iron, enabling efficient cellular iron uptake via transferrin receptor-mediated endocytosis. It plays a critical role in supporting cell proliferation, metabolism, and viability, especially in serum-free and chemically defined culture systems.\u003c\/p\u003e\n\u003cp\u003eThis recombinant protein is expressed in CHO cells and corresponds to the full-length human transferrin sequence (Val20–Pro698), ensuring proper folding and biological functionality. With high purity (≥95%) and low endotoxin levels (≤0.5 EU\/mg), this product provides consistent performance in sensitive cell culture applications. Supplied in a tag-free, lyophilized form, it is ideal for large-scale cell culture, bioprocessing, and research use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrapper\"\u003e\n\u003ctable class=\"biofargo-spec-table\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #0056b3; color: white;\"\u003e\n\u003cth colspan=\"2\" style=\"padding: 12px; text-align: left;\"\u003eProduct Details\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eCat. No.\u003c\/td\u003e\n\u003ctd\u003eY04501\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003ePurity\u003c\/td\u003e\n\u003ctd\u003e≥95%, by SDS-PAGE (under reducing (R) \u0026amp; Non-reducing conditions, visualized by Coomassie staining).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eEndotoxin\u003c\/td\u003e\n\u003ctd\u003e≤0.5 EU\/mg by the LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eBioactivity\u003c\/td\u003e\n\u003ctd\u003eMeasured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is 0.5-2 μg\/mL. Optimal concentration depends on cell type as well as the application or research objectives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eAccession\u003c\/td\u003e\n\u003ctd\u003eP02787\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eAlternative Names\u003c\/td\u003e\n\u003ctd\u003eSerotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin; TF\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eSpecies\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eExpression System\u003c\/td\u003e\n\u003ctd\u003eCHO\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eMol. Weight\u003c\/td\u003e\n\u003ctd\u003e75.2 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eExpression Region\u003c\/td\u003e\n\u003ctd\u003eVal20-Pro698\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eTag Info\u003c\/td\u003e\n\u003ctd\u003eTag free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eFormulation\u003c\/td\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eBuffer\u003c\/td\u003e\n\u003ctd\u003eSterile water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eSize\u003c\/td\u003e\n\u003ctd\u003e10μg\/100μg\/1mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eLead Time\u003c\/td\u003e\n\u003ctd\u003eProducts are in stock,but custom products may require further consultation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eReconstitution\u003c\/td\u003e\n\u003ctd\u003eIt is recommended to redissolve in sterile deionized water.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eShipping\u003c\/td\u003e\n\u003ctd\u003eThe product is shipped with blue ice.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26%\" style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eStorage \u0026amp; Stability\u003c\/td\u003e\n\u003ctd\u003e36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eHigh Purity:\u003c\/strong\u003e This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eLow Endotoxin:\u003c\/strong\u003e Endotoxin level is ≤0.5 EU\/mg, making it suitable for sensitive cell culture and biomanufacturing applications.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eIron-Saturated Form:\u003c\/strong\u003e Provided as holo-transferrin, enabling efficient iron delivery and enhanced support for cell growth and metabolism.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eOptimized for Serum-Free Culture:\u003c\/strong\u003e Ideal for use in chemically defined and serum-free media, ensuring consistent and controlled experimental conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eApplications\u003c\/h2\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 20px;\"\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eCell Culture\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eWidely used in serum-free and chemically defined media to support cell growth, viability, and proliferation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eBioprocessing\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eSuitable for large-scale cell culture and biopharmaceutical production requiring consistent iron supplementation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eCell Biology\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eUsed to study iron metabolism, transferrin receptor function, and iron-dependent cellular processes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eData Figures\u003c\/h2\u003e\n\u003cdiv style=\"text-align: center; margin-top: 20px; border: 1px solid #eee; padding: 15px; border-radius: 8px;\"\u003e\n\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1729673385909688.jpg?v=1775020868\" alt=\"Measured in a cell inhibitionn assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng\/mL. The bioactivity of Y07901 was higher than other competing products.\" style=\"max-width: 100%; height: auto;\"\u003e\n\u003cp style=\"font-size: 13px; color: #888; margin-top: 10px;\"\u003eFigure 1: Recombinant Human Transferrin (Cat. No. Y04501)  SDS-PAGE under reducing (R) \u0026amp; Non-reducing condition. The gel was stained with Y04501 SDS-PAGE.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin-top: 20px; border: 1px solid #eee; padding: 15px; border-radius: 8px;\"\u003e\n\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1729673459107314.jpg?v=1775020868\" alt=\"Measured in a cell inhibitionn assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng\/mL. The bioactivity of Y07901 was higher than other competing products.\" style=\"max-width: 100%; height: auto;\"\u003e\n\u003cp style=\"font-size: 13px; color: #888; margin-top: 10px;\"\u003eFigure 2: Recombinant Human Transferrin (Cat. No. Y04501) stimulates proliferation of MDCK cells in serum-free cell culture medium. The ED50 for this effect is 0.5-2 μg\/mL. The bioactivity of Y04501 was higher than other competing products.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin-top: 20px; border: 1px solid #eee; padding: 15px; border-radius: 8px;\"\u003e\n\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1729672900247673.jpg?v=1775020868\" alt=\"Measured in a cell inhibitionn assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng\/mL. The bioactivity of Y07901 was higher than other competing products.\" style=\"max-width: 100%; height: auto;\"\u003e\n\u003cp style=\"font-size: 13px; color: #888; margin-top: 10px;\"\u003eFigure 3: Highly consistent bioactivity between different production batches of Recombinant Human Transferrin (Cat. No. Y04501).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin-top: 20px; border: 1px solid #eee; padding: 15px; border-radius: 8px;\"\u003e\n\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/31ebc4370166f179d3bfdc3e4fceb5b0_126fec6b-fd2d-4756-afee-bfd4f56f8e06.jpg?v=1775020868\" alt=\"Measured in a cell inhibitionn assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng\/mL. The bioactivity of Y07901 was higher than other competing products.\" style=\"max-width: 100%; height: auto;\"\u003e\n\u003cp style=\"font-size: 13px; color: #888; margin-top: 10px;\"\u003eFigure 4: SEC-HPLC\u003c\/p\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eInstructions for Use\u003c\/h2\u003e\n\u003cul style=\"padding-left: 20px;\"\u003e\n\u003cli style=\"margin-bottom: 8px; text-align: left;\"\u003eDissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px; text-align: left;\"\u003ePrepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px; text-align: left;\"\u003eDo not mix with other similar products to avoid cross-reactivity and potential interference.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eCautions\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fff5f5; border-left: 5px solid #ff4d4f; padding: 15px; color: #852d2d; font-size: 14px;\"\u003e\n\u003cp style=\"margin: 0px 0px 8px; text-align: left;\"\u003e\u003cstrong\u003e• Research Use Only:\u003c\/strong\u003e This product is not suitable for clinical diagnostics or therapeutic applications.\u003c\/p\u003e\n\u003cp style=\"margin: 0px 0px 8px; text-align: left;\"\u003e\u003cstrong\u003e• Preservation:\u003c\/strong\u003e Handle according to instructions to preserve its activity and stability.\u003c\/p\u003e\n\u003cp style=\"margin: 0px; text-align: left;\"\u003e\u003cstrong\u003e• Support:\u003c\/strong\u003e Contact our technical support team if you encounter any issues or questions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"display: flex; gap: 15px; margin-top: 10px; flex-wrap: wrap;\"\u003e\u003ca style=\"display: inline-block; padding: 10px 20px; border: 2px solid #0056b3; color: #0056b3; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" target=\"_blank\"\u003e📄 Download\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"EastMabBio","offers":[{"title":"10ug","offer_id":52404391903413,"sku":"Y04501-10ug","price":25.37,"currency_code":"USD","in_stock":true},{"title":"100mg","offer_id":52404391936181,"sku":"Y04501-100mg","price":88.8,"currency_code":"USD","in_stock":true},{"title":"1g","offer_id":52404391968949,"sku":"Y04501-1g","price":501.6,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_b5ed9595-66a6-4415-a066-976dc6a0b12b.jpg?v=1775020542","url":"https:\/\/biofargo.com\/products\/recombinant-human-holo-transferrin-holo","provider":"Biofargo","version":"1.0","type":"link"}