Description

Recombinant Human Hepatocyte Growth Factor (HGF) is a multifunctional cytokine that plays a critical role in cell proliferation, motility, morphogenesis, and tissue regeneration. HGF exerts its biological effects by binding to the c-Met receptor, activating downstream signaling pathways such as PI3K/AKT, MAPK, and STAT, which are essential for cell growth and survival. It is widely involved in liver development, wound healing, angiogenesis, and tumor progression.

This recombinant protein is expressed in CHO cells and consists of both α and β chains (α chain: Gln32–Arg494; β chain: Val495–Ser728), forming the biologically active heterodimer. With high purity (≥95%) and strong bioactivity (ED50 ≤2.5 ng/mL, measured by IL-11 secretion in Saos-2 cells), this product is suitable for a wide range of applications in cell biology, oncology, and regenerative medicine. The protein is supplied in a tag-free, lyophilized form for convenient storage and reconstitution.

Specifications

Product Details
Cat. No.	Y01701
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is ≤2.5 ng/mL.
Accession	P14210
Alternative Names	Hepatocyte growth factor; HPTA; HGF; SF; Scatter factor; Hepatopoietin-A
Species	Human
Expression System	CHO
Mol. Weight	53.7 kDa ( α chain), 26 kDa ( β chain)
Expression Region	α chain: Gln 32 -Arg 494 & β chain: Val 495-Ser 728
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
✔ High Biological Activity: Demonstrates potent activity with ED50 ≤2.5 ng/mL in Saos-2 cell-based assay, ensuring strong and consistent performance.
✔ Mammalian Expression System: Expressed in CHO cells to ensure correct folding, heterodimer formation, and native-like post-translational modifications.
✔ Convenient and Reliable: Supplied in a tag-free, lyophilized form for easy reconstitution, stable storage, and compatibility with diverse research applications.

Applications

Cell Biology

Promotes cell proliferation, migration, and morphogenesis through c-Met receptor signaling pathways.

Cancer Research

Widely used in studies of tumor growth, metastasis, and HGF/c-Met signaling in oncology.

Regenerative Medicine

Involved in tissue repair, angiogenesis, and organ regeneration, especially in liver and epithelial tissues.

Data Figures

Measured in a cell inhibitionn assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2 ng/mL. The bioactivity of Y07901 was higher than other competing products.

Figure 1: Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is ≤2.5 ng/ml. The bioactivity of Y01701 was higher than the other competing product.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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Recombinant Human HGF