Description

GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor), a member of the colony-stimulating factor (CSF) family, is a cytokine that regulates hematopoiesis and immune cell function. It is secreted by various cell types, including granulocytes, dendritic cells, T cells, and B cells, and primarily modulates cell proliferation, differentiation, survival, and function through the activation of specific signaling pathways such as JAK/STAT, MAPK, and PI3K.GM-CSF promotes the proliferation and differentiation of hematopoietic progenitor cells, and stimulates the lineage-specific differentiation and expansion of granulocytes (neutrophils, eosinophils) and macrophages. It also acts synergistically with cytokines such as IL-3 and SCF to enhance multi-lineage hematopoiesis.

Specifications

Product Details
Cat. No.	Y01501
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is ≤0.2 ng/mL.
Accession	P04141
Alternative Names	Granulocyte-Macrophage Colony-Stimulating Factor; GM-CSF; Colony-Stimulating Factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF
Species	Human
Expression System	CHO
Mol. Weight	14.5 kDa
Expression Region	Ala18-Glu144
Tag Info	Tag free
Formulation	Lyophilized
Buffer	PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Stimulates proliferation and differentiation of myeloid progenitors.

Immunology

Enhances dendritic cell and macrophage activation.

Stem Cell Research

Used to drive myeloid differentiation from stem/progenitor cells.

Cell Biology

Regulates proliferation, survival, and function of myeloid cells.

Data Figures

Figure 1: Recombinant Human GM-CSF (Cat. No. Y01501) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y01501 SDS-PAGE.

Recombinant Human GM-CSF (Cat. No. Y01501) stimulates proliferation of TF-1 cells. The ED50 for this effect is ≤0.2 ng/mL. Compared with other competitors in terms of bioactivity, Y01501 is equivalent to competitor # 1 >competitor # 2>competitor # 3.

Figure 2: Recombinant Human GM-CSF (Cat. No. Y01501) stimulates proliferation of TF-1 cells. The ED50 for this effect is ≤0.2 ng/mL. Compared with other competitors in terms of bioactivity, Y01501 is equivalent to competitor # 1 >competitor # 2>competitor # 3.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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