Description

GDNF (Glial Cell Line-Derived Neurotrophic Factor) is an important neurotrophic factor belonging to the TGF-β superfamily. Initially isolated from a rat glial cell line, it has become a research focus due to its remarkable protective effects on dopaminergic neurons, showing potential therapeutic value especially in the treatment of Parkinson's disease. GDNF is also involved in the cultivation and directed differentiation of stem cells, and is often used in combination with factors such as SHH and FGF8 to induce the differentiation of embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) into dopaminergic neurons.

Specifications

Product Details
Cat. No.	Y01421H
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is ≤20 ng/mL in the presence of Recombinant Human GDNF R alpha-1.
Accession	NP_000505.1
Species	Human
Expression System	E. coli
Mol. Weight	15.2 kDa
Expression Region	S78-I211
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing 20 mM Sodium Citrate, 5% Mannitol, pH2.5.
Size	10μg/50μg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Stem Cell Research

Maintains survival and proliferation of neural stem cells and dopaminergic progenitors.

Cell Biology

Supports neuronal survival, differentiation, and axon guidance.

Data Figures

Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is ≤20 ng/mL in the presence of Recombinant Human GDNF R alpha -1.

Figure 1: Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is ≤20 ng/mL in the presence of Recombinant Human GDNF R alpha -1.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA