Description

Flt-3L (Fms-related tyrosine kinase 3 ligand) is a protein secreted by bone marrow stromal cells and other cell types. It belongs to the hematopoietic growth factor family and acts as the specific ligand for the FLT3 receptor. Upon binding to the FLT3 receptor, Flt-3L induces receptor dimerization and phosphorylation of tyrosine residues, thereby activating downstream signaling pathways such as RAS/MAPK/ERK, which influence cell fate decisions and differentiation. Flt-3L potently activates hematopoietic stem cells and is recognized as a stem cell growth factor. It acts synergistically with other growth factors—including GM-CSF, G-CSF, and IL-3—to promote the proliferation of stem cells, progenitor cells, dendritic cells, and natural killer cells.

Specifications

Product Details
Cat. No.	Y01201
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	The ED50 as determined by the dose-dependent stimulation of the proliferation of human OCI-AML5 cells is ≤ 1.0 ng/mL, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Accession	P49771
Alternative Names	Fms-Felated Tyrosine Kinase 3 Ligand; Flt3 Ligand; Flt3L; SL Cytokine; FLT3LG
Species	Human
Expression System	CHO
Mol. Weight	17.8 kDa
Expression Region	Thr27-Pro184
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Stimulates proliferation and differentiation of early hematopoietic progenitors.

Immunology

Supports dendritic cell development.

Stem Cell Research

Used to expand hematopoietic stem/progenitor cells in vitro.

Cell Biology

Regulates hematopoietic progenitor proliferation and survival.

Data Figures

Figure 1: Recombinant Human FLT-3L (Cat. No. Y01201) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y01201 SDS-PAGE.

Recombinant Human FLT-3L (Cat. No. Y01201) stimulates proliferation of OCI-AML5 cells. The ED50 for this effect is ≤1.0 ng/mL. The bioactivity of recombinant Human FLT-3L (Cat. No. Y01201) was higher than the other competing product.

Figure 2: Recombinant Human FLT-3L (Cat. No. Y01201) stimulates proliferation of OCI-AML5 cells. The ED50 for this effect is ≤1.0 ng/mL. The bioactivity of recombinant Human FLT-3L (Cat. No. Y01201) was higher than the other competing product.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA