Description

FGF-8b (Fibroblast Growth Factor 8b), an important member of the FGF family, is a splice variant of the FGF-8 isoform composed of 194 amino acids. It possesses heparin-binding capacity and can activate downstream signaling pathways by interacting with heparan sulfate proteoglycans (HSPGs). FGF-8b promotes cell proliferation, survival, and migration, playing significant roles in neural development, mesoderm formation, and carcinogenesis. It can be utilized to maintain the undifferentiated state of embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs), and works synergistically with factors such as BMP and WNT to promote the proliferation and directed differentiation of neural precursor cells.

Specifications

Product Details
Cat. No.	Y06021H
Purity	≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using NlH3T3 cells. The ED50 is ≤1.0 ng/ml.
Accession	P55075.3
Species	Human
Expression System	E. coli
Mol. Weight	22.4 kDa
Expression Region	Gln23-Arg215
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 5% Trehalose and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Stem Cell Research

Supports neural and embryonic stem cell proliferation and patterning.

Cell Biology

Involved in developmental patterning and cell differentiation, especially in neural tissues.

Data Figures

Figure 1: Measured in a cell proliferation assay using NIH3T3 cells.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human FGF-8b