Description

FGF-1 (Fibroblast Growth Factor-1 (Acidic)), also known as aFGF, is an important cell growth factor belonging to the FGF family. Its molecular core consists of a β-trefoil topological structure composed of 12 β-strands stabilized by disulfide bonds. It plays a key role in cell proliferation, differentiation, and tissue repair. FGF-1 can promote the proliferation of various cell types, such as fibroblasts, endothelial cells, neural cells, and stem cells. In serum-free culture media, when used in combination with other factors (such as TGF-β), it can inhibit stem cell differentiation and maintain their undifferentiated state.

Specifications

Product Details
Cat. No.	Y05421H
Purity	≥95%, by SDS-PAGE (under reducing (R)&Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect ≤2.0 ng/mL.
Accession	NP_001127073.1
Species	Human
Expression System	E. coli
Mol. Weight	15.8 kDa
Expression Region	F16-D155
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing 20 mM PB, 5% Trehalose, 100 mM NaCl and 0.05% Tween 80, pH6.0.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Stem Cell Research

Promotes proliferation and survival of various stem cells, including mesenchymal and endothelial stem cells.

Cell Biology

Stimulates cell proliferation, differentiation, and survival via FGFR-mediated signaling.

Data Figures

Figure 1: Measured in a cell proliferation assay using NIH3T3 cells.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human FGF-1