Description

Recombinant human erythropoietin (EPO) is a glycosylated cytokine essential for erythropoiesis, specifically promoting the proliferation and differentiation of erythroid progenitor cells (CFU-E) into mature erythrocytes.

While renal production maintains physiological EPO levels in adults, hepatic synthesis predominates during fetal and neonatal development.

Beyond its canonical role in hematopoiesis, EPO exhibits pleiotropic bioactivities, including angiogenic stimulation via vascular smooth muscle cell proliferation, neuroprotective effects under hypoxic conditions, and modulation of B-cell function.

The recombinant protein retains native conformational integrity, ensuring targeted activation of the EPO receptor (EPOR) to orchestrate tissue-specific responses without off-target interactions, making it invaluable for therapeutic applications in anemia treatment and regenerative medicine.

Specifications

Specification	Acceptance Criteria	TL-636-0050	TL-636-0100
Appearance	Freeze-dried preparation, the form should be white loose body	Compliance	Compliance
Identity	The electrophoretic bands should be consistent with the molecular weight of the product	Compliance	Compliance
Visible Particle	Should be no visible particle	Compliance	Compliance
Moisture	≤4.0%	2.0%	2.9%
pH	6.5~7.5	7.3	7.3
Purity	>90% / SDS-PAGE	Compliance	Compliance
Endotoxin	<0.1EU/μg	<0.1EU/μg	<0.1EU/μg
Mycoplasma	Should be negative	Negative	Negative
Sterility	No growth	No growth	No growth
Biological Activity	Cell proliferation assay with TF-1 cells, specific activity ≥1×10⁶ IU/mg	3.08×10⁶ IU/mg	—

Features

✔ Glycosylated, Mammalian-Derived EPO
HEK293 expression preserves native glycosylation and EPOR-specific signaling fidelity.
✔ Validated High Biological Activity
Demonstrated potency in TF-1 cell proliferation assay (≥1.0 × 10⁶ IU/mg).
✔ High Purity with Stringent QC
90% purity by SDS-PAGE with confirmed sterility, mycoplasma negativity, and low endotoxin.
✔ Consistent Lot-to-Lot Performance
Controlled moisture, pH, and activity ensure reproducibility in hematopoietic research workflows.

Applications

Erythropoiesis Research

Promotes proliferation and differentiation of erythroid progenitors (CFU-E).

T Cell-Related Studies

Applied as a functional cytokine component in immune cell research systems.

CIK Cell Research

Used in cytokine-induced killer (CIK) cell culture and functional modulation studies.

Regenerative & Angiogenesis Research

Explored for non-hematopoietic roles including angiogenesis and tissue protection under hypoxia.

Stability & Storage

Lyophilized powder: Stable for 12 months at −80 °C or 6 months at −20 °C when stored in the original sealed container under desiccant.
Reconstitution: Dissolve in sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4), maintaining a final concentration ≥100 µg/mL to prevent adsorption.
Handling: Aliquot to avoid repeated freeze–thaw cycles.

Cautions

• Research Use Only: This product is not intended for diagnostic or therapeutic use.

• Handling: Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.

• Storage: Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.

Documents

📄 TL-636 Data Sheet 📄 TL-636 SDS 📄 TL-636-0050 COA 📄 TL-636-0100 COA

Recombinant Human EPO Protein