{"product_id":"recombinant-human-ccl3-mip-1-alpha-protein","title":"Recombinant Human CCL3\/MIP-1 alpha Protein","description":"\u003cp\u003e\u003cstrong\u003eSynonym: \u003c\/strong\u003eCCL3; G0S19-1; MIP1A; SCYA3;C-C motif chemokine 3; G0\/G1 switch regulatory protein 19-1; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; PAT 464.1; SIS-beta; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein; Cleaved into: MIP-1-alpha(4-69); LD78-alpha(4-69)\u003c\/p\u003e\n\u003ch2\u003eBackground\u003c\/h2\u003e\n\u003cp\u003eCCL3 also known as macrophage inflammatory protein 1-a, is a member of the CC subfamily. It’s known that CCL3 is produced by monocytes\/macrophages, lymphocytes, neutrophils as well as immune cells such as basophils, mast cells, fibroblasts, and dendritic cells. Meanwhile, CCL3 exerts various biological effects by binding to its three cell surface receptors, including CCR1, CCR3, and CCR5. MIP-1a induces a variety of pro-inflammatory activities such as leukocyte chemotaxis, and promotes the entry of T cells into the inflammatory tissue region from blood circulation. Chemotactic CD4+ cells, CD8+ cells, natural killer cells, and dendritic cells bind to the corresponding receptors and coordinate the occurrence of immune reactions in the immune response site by migrating through vascular endothelial cells. In addition, MIP-1a is considered as a key inflammatory mediator in granuloma, asthma, T1D as well as other autoimmune diseases.\u003c\/p\u003e\n\u003ch2\u003eDescription\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eHigh quality, high purity and low endotoxin recombinant Recombinant Human CCL3\/MIP-1 alpha Protein (RP01855), tested reactivity in E. coli and has been validated in SDS-PAGE.100% guaranteed.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 90%; height: 198px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003eGene ID\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/entrez\/query.fcgi?db=gene\u0026amp;cmd=Retrieve\u0026amp;dopt=Graphics\u0026amp;list_uids=6348\"\u003e6348\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003eExpressed Host\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003eE. coli\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003eCalculated MW\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e7.78 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003eObserved MW\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e10-15 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003eTag\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003eNo-tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e≥ 90% as determined by SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003eEndotoxin\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e\u0026lt; 1 EU\/μg of the protein by LAL method.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 22px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003e\u003cstrong\u003eFormulation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 22px;\"\u003eLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003cp\u003eCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg\/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.\u003c\/p\u003e\n\u003cp\u003eAfter reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RP01855.pdf?v=1773986401\"\u003e\u003cstrong\u003eData Sheet\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS_ABclonal_recombinant_protein_No_hazards_1.pdf?v=1773986401\"\u003e\u003cstrong\u003eSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"abclonal","offers":[{"title":"10μg","offer_id":52368927162549,"sku":"RP01855-10μg","price":145.0,"currency_code":"USD","in_stock":true},{"title":"20μg","offer_id":52368927195317,"sku":"RP01855-20μg","price":218.0,"currency_code":"USD","in_stock":true},{"title":"50μg","offer_id":52368927228085,"sku":"RP01855-50μg","price":363.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":52368927260853,"sku":"RP01855-100μg","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_0c89cbe7-7c80-4de2-882a-0a2f806d9278.jpg?v=1773985556","url":"https:\/\/biofargo.com\/products\/recombinant-human-ccl3-mip-1-alpha-protein","provider":"Biofargo","version":"1.0","type":"link"}