Description

BMP-9 (Bone Morphogenetic Protein 9), also known as Growth Differentiation Factor-2 (GDF-2), belongs to the TGF-β superfamily. It signals through heteromeric complexes composed of type I and type II BMP receptors and has been identified in recent years as a bone morphogenetic protein with exceptionally potent osteoinductive activity. BMP-9 can induce osteogenic differentiation in various cell types, including osteoblasts, osteoprogenitor cells, and other committed stem cells.

Specifications

Product Details
Cat. No.	Y10801
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is ＜2.0 ng/mL.
Accession	Q9UK05
Species	Human
Expression System	HEK293
Mol. Weight	12.1 kDa (monomer)
Expression Region	Ser320-Arg429
Tag Info	Tag free
Formulation	Lyophilized
Buffer	0.085% TFA, 30% ACN, 5% Mannitol, pH2.5.
Size	50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in 4 mM HCl.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Stem Cell Research

Potent inducer of osteogenic differentiation in MSCs and progenitors.

Cell Biology

Controls osteogenesis, angiogenesis, and metabolic regulation through SMAD signaling.

Data Figures

Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is ＜2.0 ng/mL. The activity of recombinant Human BMP-9 (Cat. No. Y10801) is higher than the other competing product.

Figure 1: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is ＜2.0 ng/mL. The activity of recombinant Human BMP-9 (Cat. No. Y10801) is higher than the other competing product.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human BMP-9