Description

BMP-2 (Bone Morphogenetic Protein 2), a member of the transforming growth factor-beta (TGF-β) superfamily, is synthesized in vivo as a precursor protein. Through proteolytic cleavage that removes the signal peptide and propeptide, it yields a mature peptide consisting of 114 amino acid residues. This mature peptide further forms homodimers or heterodimers via dimerization, thereby acquiring full biological activity. BMP-2 stimulates DNA synthesis and cell replication, promoting the directed differentiation of mesenchymal cells into osteoblasts. As a key factor inducing bone and cartilage formation in vivo, BMP-2 is expressed during limb growth, endochondral ossification, early fracture healing, and cartilage repair, playing a critical role in embryonic skeletal development and regenerative repair.

Specifications

Product Details
Cat. No.	Y00421H
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤100 EU/mg by the LAL method.
Bioactivity	Measured by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is ＜0.5 ug/ml.
Accession	P12643
Species	Human
Expression System	E. coli
Mol. Weight	12.9 kDa (monomer)
Expression Region	Gln283-Arg396
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm filtered solution containing 0.1 M Acetic acid, 5% Trehalose, pH 3.0.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in 4 mM HCl.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Hematology

Promotes osteogenic differentiation of hematopoietic niche stromal cells.

Immunology

Modulates immune cell maturation and inflammatory responses indirectly.

Stem Cell Research

Induces mesenchymal stem cell osteogenic differentiation and supports tissue engineering.

Cell Biology

Key regulator of bone formation, cell differentiation, and morphogenesis via SMAD1/5/8.

Data Figures

Figure 1: Measured by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is ＜0.5 ug/ml.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human BMP-2