Description

As a core member of the FGF family, bFGF selectively binds to FGFR1c/2c/3c/4 receptor subtypes via its β-trefoil structure, activating the Ras-MAPK and PI3K-AKT signaling axes to regulate critical physiological processes, including cell proliferation, migration, differentiation, and angiogenesis.

In cell therapy applications, bFGF serves as an essential component of serum-free stem cell culture systems, sustaining the undifferentiated state of human pluripotent stem cells (hPSCs) through sustained ERK1/2 pathway activation while stabilizing Nanog/Oct4 expression. It further synergizes with BMP inhibitors to prevent spontaneous germ layer differentiation.

This recombinant thermostable human basic fibroblast growth factor (bFGF-HS) is engineered via an AI-driven protein design platform and produced in E. coli as a tag-free protein. While preserving the native heparin-binding domain (residues 128–144) and biological activity, the optimized sequence significantly enhances thermal stability and in vitro potency.

Compared to wild-type bFGF, this mutant exhibits an extended half-life in culture systems, reducing medium replenishment frequency and contamination risks, making it particularly suitable for large-scale stem cell culture, tissue engineering, and regenerative medicine research.

Specifications

Cytokines Details
Synonyms	FGF2, FGFB, FGF basic, HBGF-2
Accession	UniProt P09038-1
Expressed Region	Gly59–Ser210 with an N-terminal methionine and several site mutations
Tag	Tag-free
Expression System	E. coli
Predicted Molecular Weight	17.0 kDa
Purity	> 90% as determined by SDS-PAGE
Endotoxin	< 0.1 EU per 1 μg of protein (LAL method)
Biological Activity	Measured by HUVEC cell proliferation assay, corresponding to a specific activity of ≥ 1.0 × 10⁶ IU/mg
Formulation	Lyophilized from sterile PBS (pH 7.4), typically supplemented with 6% mannitol as a stabilizing protectant

Features

✔ Heat-Stable Engineered Variant
AI-designed mutations significantly enhance thermal stability while preserving native bFGF activity.
✔ Sustained ERK/AKT Signaling
Provides prolonged FGFR activation to support continuous cell proliferation and survival.
✔ High Biological Activity
Validated by HUVEC proliferation assay, ensuring reliable potency in serum-free culture systems.
✔ Excellent Batch Stability
Consistent performance across batches reduces variability in long-term cell culture workflows.

Applications

Human iPSC Maintenance

Maintains pluripotency by stabilizing Nanog/Oct4 expression and preventing spontaneous differentiation.

Mesenchymal Stem Cell (MSC) Expansion

Promotes robust MSC proliferation and migration in serum-free conditions.

Large-Scale Stem Cell Culture

Extended half-life reduces media change frequency and contamination risk.

Regenerative Medicine & Tissue Engineering Research

Supports angiogenesis, cell migration, and growth factor–dependent tissue models.

Stability & Storage

√ Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
√ Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration ≥ 100 µg/mL to prevent adsorption.
√ Handling: Aliquot to avoid repeated freeze-thaw cycles.

Cautions

• Research Use Only: This product is not intended for diagnostic or therapeutic use.

• Handling: Follow recommended storage and handling instructions to maintain product stability.

• Support: Contact technical support if assistance is required.

Documents

📄 TL-787 Data Sheet 📄 TL-787 SDS 📄 TL-787 SDS

Recombinant Human bFGF Protein (Heat Stable)