Description

Brain-Derived Neurotrophic Factor (BDNF) is one of the most widely distributed and extensively studied neurotrophic factors in the mammalian brain.

BDNF is secreted by glutamatergic neurons and glial cells in the brain.

It regulates normal brain functions by binding to and activating TrkB.

BDNF–TrkB signaling promotes the differentiation of cortical progenitor cells into neurons and participates in adult neurogenesis in the hippocampus.

BDNF modulates synaptic transmission and long-term potentiation (LTP) in the hippocampus and other brain regions.

Dysregulation of BDNF signaling is associated with multiple disorders, including Alzheimer’s disease (AD), depression, and schizophrenia.

Specifications

Parameter	Specification
Expression Host	E. coli
Synonyms	Abrineurin; BDNF; ANON2; BULN2
Protein Sequence	Amino acid sequence: His129–Arg247 of human BDNF (UniProt ID: P23560-1)
Molecular Weight	Predicted molecular weight is 13.51 kDa
Purity	>95%, analyzed by SDS-PAGE and HPLC
Endotoxin	<0.01 EU/μg
Purification Method	Chromatographic purification
Appearance	White lyophilized powder
Activity	Refer to COA for details

Features

✔ Recombinant Human BDNF Protein (E. coli Expression)
Produced in E. coli and purified through chromatographic methods, ensuring high purity and consistent protein quality suitable for neuronal research applications.
✔ Defined Human BDNF Functional Fragment
Corresponds to the His129–Arg247 region of human BDNF with a predicted molecular weight of approximately 13.51 kDa.
✔ Ultra-Low Endotoxin Preparation
Endotoxin level <0.01 EU/μg, supporting sensitive cell culture systems and minimizing inflammatory interference during neuronal studies.
✔ High-Purity Lyophilized Protein Format
Purity >95% verified by SDS-PAGE and HPLC, supplied as a white lyophilized powder for stable storage and reproducible experimental performance.

Applications

Neuronal Differentiation Research

BDNF activates TrkB signaling to promote differentiation of cortical progenitor cells into neurons.

Adult Neurogenesis Studies

Supports investigation of hippocampal neurogenesis and neuronal maturation in brain development models.

Synaptic Plasticity & LTP Research

Widely used to study synaptic transmission and long-term potentiation (LTP) mechanisms in hippocampal and neural circuit models.

Stem Cell Culture Systems

Applicable for stem cell culture environments including iPSC and MSC research systems.

Storage

Store below -20°C

Instructions

Reconstitute in distilled water to a concentration >100 μg/mL. Following reconstitution, the product is stable for 3-6 months when stored at -80°C, or for 2-7 days when stored at 2-8°C.

Precautions

This product is only suitable for in vitro cell culture and cannot be used directly in clinical treatment.

Documents

📄 TL-770 Data Sheet 📄 TL-770 SDS 📄 TL-770 COA

Recombinant Human BDNF Protein