Description

BDNF (Brain-Derived Neurotrophic Factor) is a neurotrophic factor belonging to the neurotrophin family, characterized by a conserved cysteine knot structure and sharing homology with other neurotrophins such as NGF and NT-3. It is widely expressed in the central and peripheral nervous systems, with particularly high concentrations in brain regions like the hippocampus and cerebral cortex. BDNF plays crucial roles in neural growth, survival, synaptic plasticity, and cognitive function. It inhibits apoptotic pathways and enhances neuronal survival under conditions of serum deprivation or stress. When combined with factors like retinoic acid, BDNF can induce the differentiation of neural stem cells (NSCs) or PC12 cells into neurons.

Specifications

Product Details
Cat. No.	Y00201
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	The activity is determined by the dose-dependent proliferation of C6 cell sand is typically between 0.5-1.5 μg/mL. Corresponding to a specific activity of 1 x 103 units/mg.
Accession	P23560
Species	Human
Expression System	CHO
Mol. Weight	13.5 kDa
Expression Region	His129-Arg247
Tag Info	Tag Free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm filtered solution containing PBS, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Stem Cell Research

Supports survival and differentiation of neural stem cells.

Cell Biology

Regulates neuronal survival, differentiation, and synaptic plasticity.

Data Figures

The activity is determined by the dose-dependent proliferation of C6 cell sand is typically between 0.5-1.5 μg/mL. Corresponding to a specific activity of 1 x 103 units/mg.

Figure 1: The activity is determined by the dose-dependent proliferation of C6 cell sand is typically between 0.5-1.5 μg/mL. Corresponding to a specific activity of 1 x 103 units/mg.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human BDNF