Description

Transferrin is an important glycoprotein composed of two domains, each containing an iron-binding site—one at the N-terminus and one at the C-terminus. Each site can bind one Fe³⁺ ion with the cooperative involvement of one HCO₃⁻ ion. Its primary role is to transport and regulate iron metabolism, delivering iron to cells in a safe manner that avoids the toxicity associated with free iron ions. Iron is an essential cofactor for haemoglobin synthesis, electron transport chain components (such as cytochromes), and DNA synthesis enzymes, making it particularly critical for highly proliferative cells like cancer cells and stem cells. As a key additive in serum-free culture media, transferrin can substitute for serum as an iron source, ensuring cell survival and growth.

Specifications

Product Details
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is ≤5 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
Accession	P02787
Alternative Names	Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin; TF
Species	Human
Expression	CHO
Mol. Weight	75.2 kDa
Expression Region	Val20-Pro698
Tag Info	Tag free
Formulation	Lyophilized
Buffer	sterile water
Size	100mg/1g
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at 2°C to 8°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Stem Cell Research

Supplies iron in culture media, supporting stem cell proliferation.

Cell Biology

Iron transport, supports metabolism and proliferation in vitro.

Data Figures

Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is ≤5 μg/mL. The activity of Y04504 was higher than other competing products.

Figure 1: Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is ≤5 μg/mL. The activity of Y04504 was higher than other competing products.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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Recombinant Human Apolactoferrin (APO)