Description

Activin A is a dimeric glycoprotein belonging to the TGF-β superfamily. It plays crucial roles in various physiological processes, including embryonic differentiation, trophoblast invasion during early pregnancy, and neuroprotection under hypoxic conditions.

Additionally, Activin A is involved in bone formation and regeneration, contributes to joint inflammation in rheumatoid arthritis, and mediates pathogenic mechanisms in the respiratory system.

Activin A signals through a complex of two type I and two type II serine/threonine kinase receptors. Upon ligand binding, these receptors activate their kinase activity and phosphorylate the intracellular mediators SMAD2 and SMAD3.

The phosphorylated SMADs then form a heteromeric complex with SMAD4. This complex translocates to the nucleus, where it regulates the transcription of target genes.

Specifications

Parameter	Specification
Expression Host	HEK293 cells
Synonyms	Activin A; INHBA
Protein Sequence	Amino acid sequence: Gly311–Ser426 of human Activin A (UniProt ID: P08476)
Molecular Weight	Predicted molecular weight is 12.97 kDa
Purity	>95%, analyzed by SDS-PAGE under reducing conditions
Endotoxin	<0.1 EU/μg
Purification Method	Chromatographic purification
Appearance	White lyophilized powder
Activity	Refer to COA for details

Features

✔ HEK293-Expressed Activin A (Gly311–Ser426)
Recombinant human Activin A corresponding to Gly311–Ser426 of human INHBA, expressed in HEK293 cells with a predicted molecular weight of 12.97 kDa.
✔ High Purity Chromatographic Preparation
Purity >95% determined by SDS-PAGE under reducing conditions following chromatographic purification.
✔ Low Endotoxin Profile
Endotoxin level <0.1 EU/μg supporting sensitive cell culture applications.
✔ Defined Lyophilized Protein Format
Supplied as a white lyophilized powder with consistent electrophoretic migration (~14–15 kDa reducing conditions).

Applications

MSC Differentiation Studies

Used to regulate mesenchymal stem cell differentiation and signaling processes in vitro.

iPSC Culture & Lineage Specification

Supports pluripotent stem cell signaling pathways involved in differentiation and developmental modeling.

SMAD2/SMAD3 Signaling Research

Activates type I and type II serine/threonine kinase receptor complexes leading to SMAD2/3 phosphorylation and downstream transcriptional regulation.

Developmental Biology Models

Applied in studies of embryonic differentiation, trophoblast invasion, and hypoxia-related cellular responses.

Instructions

Centrifuge before reopening the tube. Dissolve the lyophilized protein in 4 mM HCl. It is recommended to reconstitute the protein to a concentration greater than 100 μg/mL. After reconstitution, the protein can be stored at -80°C for 3-6 months and at 2-8℃ for 2-7 days. For optimal storage, aliquot the protein and minimize freeze-thaw cycles.

Precautions

This product is only suitable for in vitro cell culture and cannot be used directly in clinical treatment.

Storage

Store below -20°C

Document

📄 TL-910 Data Sheet 📄 TL-910 SDS 📄 TL-910 COA

Recombinant Human Activin A Protein