Background

4-1BB ligand (4-1BBL), also known as CD137L or TNFSF9, is a member of the tumor necrosis factor (TNF) family expressed on activated antigen-presenting cells. Its receptor, 4-1BB, is a member of the TNF receptor family expressed on activated CD4 and CD8 T cells. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4+ and CD8+ T cells. T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response. Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

Specifications

Protein sequence	A DNA sequence encoding the human 4-1BB Ligand (NP_003802.1) was expressed with a Fc-tag at the C-terminus.
Expression Host	HEK293 cells
QC Testing Purity	> 90 % as determined by SDS-PAGE
Activity	Measured by its ability to induce IL-8 secretion by PBMC. The ED₅₀ for this effect is ≤ 10 ng/mL.
Endotoxin Level	< 0.1 EU per 1 μg of the protein by the LAL method.
Molecular Mass	The recombinant human 4-1BB Ligand protein consists of 467 amino acids and predicts a molecular mass of 51 kD.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization.
Stability & Storage	24 months at 2 ℃ to 8 ℃ in lyophilized state. 6 months at -20 ℃ under sterile conditions after reconstitution. 12 months at -80 ℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100 μg/mL. Avoid repeated freeze-thaw cycles.

References

4-1BB Ligand Signaling to T cells Limits T cell Activation So-Young Eun, Seung-Woo Lee, Yanfei Xu, Michael Croft J Immunol. Author manuscript; available in PMC 2016 Jan 1.Published in final edited form as: J Immunol. 2015 Jan 1;194(1): 134–141. Published online 2014 Nov 17. doi: 10.4049/jimmunol.1401383.
4-1BB ligand activates bystander dendritic cells to enhance immunisation in trans Douglas C Macdonald, Alastair Hotblack, Saniath Akbar, Gary Britton, Mary K Collins, William C Rosenberg J Immunol. Author manuscript; available in PMC 2015 May 15.Published in final edited form as: J Immunol. 2014 Nov 15; 193(10): 5056–5064. Published online 2014 Oct 10.doi:10.4049/jimmunol.1301723.
A functional recombinant human 4-1BB ligand for immune co-stimulatory therapy of cancer Marcia Meseck, Tiangui Huang, Ge Ma, George Wang, Shu-Hsia Chen, Savio LC Woo J Immunother. Author manuscript; available in PMC 2012 Mar 1.Published in final edited form as: J Immunother. 2011 Mar; 34(2): 175–182. doi: 10.1097/CJI.0b013e318206dac1.

Document

Recombinant Human 4-1BB Ligand Protein

Recombinant Human 4-1BB Ligand Protein_SDS