Description

The recombinant human 4-1BB Ligand-Fc fusion protein is a functional dimeric immunomodulator produced in HEK-293 expression systems, featuring a C-terminal human IgG1 Fc domain to enhance structural stability and receptor clustering efficiency.

As a TNF superfamily member (TNFSF9/CD137L), it activates NF-κB and MAPK signaling pathways through binding to 4-1BB (CD137/TNFRSF9) on T cells, delivering critical costimulatory signals that drive T-cell proliferation, IFN-γ/IL-2 secretion, and expansion of effector memory T-cell subsets, while suppressing activation-induced cell death (AICD) via upregulation of Bcl-xL.

In cell therapy applications, this fusion protein serves as a pivotal component in ex vivo expansion systems, mimicking antigen-presenting cell (APC) costimulatory signals to significantly enhance CAR-T cell clonal expansion and persistence.

It synergizes with CD28 signaling to optimize T-cell metabolic reprogramming and boost effector functions of tumor-infiltrating lymphocytes (TILs), providing functional support for solid tumor immunotherapy.

The Fc domain design not only extends serum half-life but also ensures high-affinity trimeric engagement with 4-1BB receptors through spatial conformation optimization, enabling sustained signal activation.

Specifications

Parameter	Specification	Results
Appearance	Freeze-dried preparation, the form should be white loose body	Compliance
Identity	The electrophoretic bands should be consistent with the molecular weight of the product	Compliance
Visible Particle	Should be no visible particle	Compliance
Moisture	≤4.0%	1.4%
pH	6.5~7.5	7.3
Purity	>90.0%	Compliance
Endotoxin	<0.1EU/μg	<0.1EU/μg
Mycoplasma	Should be negative	Negative
Sterility	No growth	No growth
Biological Activity	Each hole is covered by 100μL Recombinant human 4-1BB (His tag) protein with a concentration of 1μg/mL; the ability of 4-1BB Ligand (Fc tag) to bind to 4-1BB (His tag) protein was determined by ELISA, ED50 ≤ 50ng/mL	20.53ng/mL

Features

✔ Mammalian-Expressed Fc Fusion Design
Recombinant human 4-1BB Ligand (Ala50–Glu254) expressed in HEK293 cells with a C-terminal human IgG1 Fc fragment, forming a dimeric fusion protein with a predicted molecular weight of 51.0 kDa.
✔ High Purity and Identity Verification
Purity >90% as determined by SDS-PAGE, with electrophoretic bands consistent with the expected molecular weight and no visible particles detected.
✔ Low Endotoxin and Sterility Assurance
Endotoxin level <0.1 EU per 1 µg protein (LAL assay), mycoplasma negative, and sterility confirmed with no microbial growth.
✔ Validated 4-1BB Binding Activity
Binds immobilized recombinant human 4-1BB/His Tag (1 µg/mL, 100 µL/well) in functional ELISA with ED₅₀ ≤50 ng/mL (measured 20.53 ng/mL).

Applications

T Cell Costimulatory Activation

Engages 4-1BB (CD137/TNFRSF9) on T cells to activate NF-κB and MAPK pathways, promoting T-cell proliferation and IFN-γ/IL-2 secretion.

CAR-T Ex Vivo Expansion Support

Functions as a costimulatory component in ex vivo expansion systems to enhance CAR-T cell clonal expansion and persistence.

Effector Memory T Cell Modulation

Drives expansion of effector memory T-cell subsets and suppresses activation-induced cell death via Bcl-xL upregulation.

TIL Functional Enhancement Research

Synergizes with CD28 signaling to optimize T-cell metabolic reprogramming and enhance effector functions of tumor-infiltrating lymphocytes.

Stability & Storage

Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
Reconstitution: Dissolve in sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4), maintaining final concentration ≥100 µg/mL to prevent adsorption.
Handling: Aliquot to avoid repeated freeze-thaw cycles.

Cautions

• Research Use Only: This product is not intended for diagnostic or therapeutic use.

• Handling: Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.

• Storage: Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.

Documents

📄 GMP-TL695 Data Sheet 📄 GMP-TL695 SDS 📄 GMP-TL695 COA

Recombinant Human 4-1BB Ligand Protein