Description

β-NGF (β-Nerve Growth Factor) is a neurotrophin belonging to the neurotrophic factor family. While structurally similar to other neurotrophins such as BDNF, NT-3, and NT-4/5, β-NGF specifically binds to the TrkA receptor (high affinity) and the p75NTR receptor (low affinity). In in vitro cultures, it primarily supports the survival, differentiation, and functional maintenance of specific neural populations, including sympathetic neurons, sensory neurons (e.g., dorsal root ganglion cells), and central cholinergic neurons (e.g., basal forebrain neurons). In downstream applications, β-NGF can be used in combination with stem cell cultures to induce the directed differentiation of neural precursor cells.

Specifications

Product Details
Cat. No.	Y06941H
Purity	≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is ＜10 ng/mL.
Accession	P01138
Species	Human
Expression System	HEK293
Mol. Weight	13.4 kDa
Expression Region	Ser122-Ala241
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22 μm-filtered solution containing 20 mM Sodium acetate, 150 mM NaCl, 5% Mannitol, 0.01% Tween 80, pH5.5.
Size	10μg/50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Immunology

Modulates immune cell survival and mast cell activation indirectly.

Stem Cell Research

Promotes survival and differentiation of neuronal progenitors.

Cell Biology

Regulates neuron survival, differentiation, and neurite outgrowth.

Data Figures

Figure 1: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is ＜10 ng/mL.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

📄 Download COA

Recombinant Human β-NGF