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Key features and details:
- Expression System:scherichia coli
- Purity/method:95% as determined by SDS-PAGE. Ni-NTA chromatography.
- Fusion tag:ag-free
- Application:WB, ELISA, SDS-PAGE, Cell culture, Elisa
CXCL1/GROa act as a growth factor for melanoma cells. It is also a chemotaxin for neutrophils. Like other alpha chemokines, this protein is potent neutrophil attractant and activator and is also affect basophils. Clinical research show that CXCL1 protein may be a therapeutic target as well as a diagnostic marker in ovarian cancer.
Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2. The ED50 for this effect is <15 ng/mL.
Endotoxin level:<0.1 EU per 1 ug of the protein by the LAL method.
Calculated Molecular Weight:7.45 kDa
Formulation:The protein was lyophilized from a solution containing 50 mM Tris and 150 mM NaCl, pH 8.5.
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Stability and Storage:
Lyophilized protein should be stored at -20 degrees Celsius for 1 year. Upon reconstitution, store at 2 degrees Celsius to 8 degrees Celsius for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20 degrees Celsius or -80 degrees Celsius for 3-6 months.
SDS-PAGE Image Name:mouse CXCL1
Datasheets and documents:
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