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Key features and details:
- Expression System:scherichia coli
- Purity/method:98% as determined by SDS-PAGE. Ni-NTA chromatography
- Fusion tag:is-tag at the N-terminus
- Application:WB, ELISA, SDS-PAGE, Cell culture, Elisa
CXCL16 belongs to the CXC chemokine family and conducts signaling through the CXCR6 receptor. CXCL16 may act as an attractant of lymphocyte subsets during inflammation process and may facilitate certain immune responses. Among six cysteine residues of the chemokine domain, there are four highly conserved cysteine residues characteristic of CXC chemokines. The CXCL16 gene encodes a 273 amino acid polypeptide, which contains a 29 amino acid cytoplasmic domain and a 20 amino acids transmembrane sequence.
NQGSVAGSCSCDRTISSGTQIPQGTLDHIRKYLKAFHRCPFFIRFQLQSKSVCGGSQDQWVRELVDCFERKECGTGHGKSFHHQKHLP with polyhistidine tag at the N-terminus.
Measure by its ability to chemoattract BaF3 cells transfected with mouse CXCR6 The ED50 for this effect is <3 ng/mL.
Endotoxin level:<0.1 EU per 1 ug of the protein by the LAL method.
Calculated Molecular Weight:10.74 kDa
Formulation:The protein was lyophilized from a solution containing 1X PBS, pH 7.4.
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Stability and Storage:
Lyophilized protein should be stored at -20 degrees Celsius for 1 year. Upon reconstitution, store at 2 degrees Celsius to 8 degrees Celsius for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20 degrees Celsius or -80 degrees Celsius for 3-6 months.
SDS-PAGE Image Name:mouse CXCL16
Datasheets and documents:
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