Recombinant CD40L,Human,AF

Key features and details:

• Expression System:Escherichia coli
• Purity/method:>95% as determined by SDS-PAGE. Ni-NTA chromatography.
• Fusion tag:His-tag at the C-terminus
• Application: WB, ELISA, SDS-PAGE, Cell culture, Elisa

Product Description:

Protein Description:

CD40, belonging to the TNF receptor family, is a cell surface protein generally expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and on T cells at low levels. The membrane-anchored CD40 Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. CD40 signaling plays a significant role immunoglobulin (Ig) class switching, and is critical for the proliferation and differentiation of B cells. A disease "immunodeficiency with hyper-IgM", characterized by failure to produce IgG, IgA and IgE due to failure expression of CD40L. The soluble form of CD40L is produced in vivo by an intracellular proteolytic processing of the entire TNF homologous region (18 kDa protein) of CD40L.

Protein Accession: P29965

Gene ID: 959

Species: Human

Expression Sequence:

MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL with polyhistidine tag at the C-terminus

Activity:

Measure by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is <5 ng/mL.

Endotoxin level: <0.1 EU per 1 ug of the protein by the LAL method.

Calculated Molecular Weight: 16.99 kDa

Formulation: The protein was lyophilized from a solution containing 1X PBS, pH 8.0.

Reconstitution:

It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Shipping: Blue Ice

Stability and Storage:

Lyophilized protein should be stored at -20 degrees Celsius for 1 year. Upon reconstitution, store at 2 degrees Celsius to 8 degrees Celsius for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20 degrees Celsius or -80 degrees Celsius for 3-6 months.

Category: Cytokines

SDS-PAGE Image Name: human CD40L

Datasheets and documents: