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Key features and details:
- Expression System:Escherichia coli
- Purity/method:>98% as determined by SDS-PAGE. Ni-NTA chromatography
- Fusion tag:tag-free
- Application: WB, ELISA, SDS-PAGE, Cell culture, Elisa
Product Description:
Protein Description:
CCL3 is belonging to the CC chemokine family. CCL3 participates in activating and recruiting cells, such as lymphocytes, monocytes, and granulocytes during acute inflammation. In addition, CCL3 can enhance IFN-’� secretion from activated T cells and thus induces Th1 response, thereby to regulating leukocyte migration. It is reported that CCL3 is involved in susceptibility of HIV infection and disease progression of AIDS.
Protein Accession: P10147
Gene ID: 6348
Species: Human
Expression Sequence:
ADTPTACCFSYTSRQIPQNFIADYFETSSQCSKPGVIFLTKRSRQVCADPSEEWVQKYVSDLELSA.
Activity:
Measure by its ability to chemoattract BaF3 cells transfected with human CCR5. The ED50 for this effect is <10 ng/mL.
C3
Endotoxin level: <0.1 EU per 1 ug of the protein by the LAL method.
Calculated Molecular Weight: 7.43 kDa
Formulation: The protein was lyophilized from a solution containing 50 mM Tris and 150 mM NaCl, pH 8.5.
Reconstitution:
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping: Blue Ice
Stability and Storage:
Lyophilized protein should be stored at -20 degrees Celsius for 1 year. Upon reconstitution, store at 2 degrees Celsius to 8 degrees Celsius for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20 degrees Celsius or -80 degrees Celsius for 3-6 months.
Category: Cytokines
SDS-PAGE Image Name: human CCL3
Datasheets and documents:
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