Description

Recombinant Bovine Transforming Growth Factor Beta 3 (TGF-β3) is a multifunctional cytokine that plays a key role in regulating cell proliferation, differentiation, migration, and immune responses. As a member of the TGF-β superfamily, TGF-β3 is involved in embryonic development, tissue regeneration, and extracellular matrix production. It signals through TGF-β receptors to activate SMAD-dependent and independent pathways.

This recombinant protein is expressed in CHO cells and corresponds to the mature region of bovine TGF-β3 (Arg337–Ser452), ensuring proper folding and biological activity. With high purity (≥95%) and strong bioactivity (ED50 ≤0.3 ng/mL in TF-1 cell inhibition assay), this product is suitable for research in cell biology, immunology, and tissue engineering. The protein is supplied in a tag-free, lyophilized form for convenient handling and storage.

Specifications

Product Details
Cat. No.	Y07901
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤10 EU/mg by the LAL method.
Bioactivity	Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.3 ng/mL.
Accession	A6QP91
Alternative Names	Transforming growth factor beta-3; TGFB3; TGF-beta-3; Latency-associated peptide; LAP
Species	Bovine
Expression System	CHO
Mol. Weight	13 kDa
Expression Region	Arg337-Ser452
Tag Info	Tag free
Formulation	Lyophilized
Buffer	Lyophilized from a 0.22-μm filtered solution containing 100 mM Glycine, 150 mM NaCl, 5% mannitol and 0.01% Tween 80, pH 4.0.
Size	10 μg / 50 μg / 1 mg / 20 mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution; Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
✔ High Biological Activity: Exhibits strong inhibitory activity with an ED50 ≤0.3 ng/mL in TF-1 cell-based assay, ensuring consistent performance.
✔ Mammalian Expression System: Produced in CHO cells to ensure correct protein folding and native-like post-translational modifications.
✔ Stable Formulation: Lyophilized with optimized buffer system to maintain protein stability during storage and transport.

Applications

Tissue Engineering

Involved in extracellular matrix production and tissue repair, widely used in regeneration studies.

Immunology

Modulates immune responses and plays a role in immune suppression and inflammation regulation.

Cell Biology

Regulates cell proliferation, differentiation, and apoptosis via TGF-β signaling pathways.

Data Figures

Figure 1: Measured in a inhibition assay using TF-1 cells in the presence of IL4.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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Recombinant Bovine TGF-β3