Description

FGF-2 (Fibroblast Growth Factor-2 (Basic)), also known as bFGF, is one of the earliest recognized members of the FGF family. Secreted by endothelial cells, smooth muscle cells, and macrophages, FGF-2 acts on a variety of cell types. As a crucial regulator of cell growth and differentiation, FGF-2 possesses biological activities such as pro-angiogenic, proliferative, chemotactic, and migratory effects, playing a vital role in processes of cell differentiation and organismal development.

Specifications

Product Details
Cat. No.	Y00331
Purity	≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining).
Endotoxin	≤200 EU/mg by the LAL method.
Bioactivity	Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤2.0 ng/mL.
Accession	P03969
Alternative Names	Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB
Species	Bovine
Expression System	E. coli
Mol. Weight	17.4 kDa
Expression Region	Pro10-Ser155
Tag Info	Tag free
Buffer	Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, 250 mM NaCl, 5% Mannitol and 0.01% Tween 80, pH7.4.
Size	50μg/1mg
Lead Time	Products are in stock, but custom products may require further consultation.
Reconstitution	It is recommended to redissolve in sterile deionized water.
Shipping	The product is shipped with blue ice.
Storage & Stability	36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Features

✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.

Applications

Used for developing species-specific cell agriculture solutions and veterinary research applications.

Data Figures

Recombinant Bovine bFGF (Cat. No. Y00331) stimulates proliferation of NIH3T3 cells. The ED50 for this effect is ≤ 2.0 ng/mL. The bioactivity of Recombinant Bovine bFGF (Cat. No. Y00331) was equivalent to other competing products.

Figure 1: Recombinant Bovine bFGF (Cat. No. Y00331) stimulates proliferation of NIH3T3 cells. The ED50 for this effect is ≤ 2.0 ng/mL. The bioactivity of Recombinant Bovine bFGF (Cat. No. Y00331) was equivalent to other competing products.

Instructions for Use

Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
Do not mix with other similar products to avoid cross-reactivity and potential interference.

Cautions

• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.

• Preservation: Handle according to instructions to preserve its activity and stability.

• Support: Contact our technical support team if you encounter any issues or questions.

Documents

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