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Key features and details:
- Expression System:Escherichia coli
- Purity/method:>98% as determined by SDS-PAGE. Ni-NTA chromatography
- Fusion tag:His-tag at the N-terminus
- Application: WB, ELISA, SDS-PAGE, Cell culture, Elisa
Bone morphogenetic protein 8B is a protein that in humans is encoded by the BMP8B gene. The protein encoded by this gene is a member of the TGF-ض superfamily. It has close sequence homology to BMP7 and BMP5 and is believed to play a role in bone and cartilage development. BMP-8b, in contrast, is required for both the initiation and maintenance of spermatogenesis.
Protein Accession: P34820
Gene ID: 656
AVRPLRRRQPKKSNELPQANRLPGIFDDVHGSHGRQVCRRHELYVSFQDLGWLDWVIAPQGYSAYYCEGECSFPLDSCMNATNHAILQSLVHLMMPDAVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCH with polyhistidine tag at the N-terminus.
Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is <21.8 ng/mL.
Endotoxin level: <0.01 EU per 1 ug of the protein by the LAL method.
Calculated Molecular Weight: 16.48 kDa
Formulation: The protein was lyophilized from a solution containing 20 mM sodium citrate, 0.2 MNaCl, pH 3.5.
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
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Stability and Storage:
Lyophilized protein should be stored at -20 degrees Celsius for 1 year. Upon reconstitution, store at 2 degrees Celsius to 8 degrees Celsius for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20 degrees Celsius or -80 degrees Celsius for 3-6 months.
SDS-PAGE Image Name: human BMP-8b
Datasheets and documents:
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