For Research Use Only (RUO)

This product is intended for research use only and is not intended for diagnostic or therapeutic use.

Description

Cell-free DNA (cfDNA) refers to short, fragmented DNA molecules that are released into the bloodstream and other bodily fluids following cell death (apoptosis or necrosis). These fragments, typically ranging from 160 to 200 base pairs in length, provide a non-invasive window into the physiological and pathological state of the body.

The analysis of cfDNA, particularly the subset derived from tumors known as circulating tumor DNA (ctDNA), has revolutionized diagnostic medicine. Its key applications encompass:

Non-Invasive Prenatal Testing (NIPT): The detection of fetal cfDNA in maternal plasma allows for the early screening of chromosomal aneuploidies without any risk to the fetus.
Liquid Biopsy in Oncology: This is a transformative approach for cancer management. It enables early cancer detection, identification of targetable mutations for personalized therapy, real-time monitoring of treatment response, and early detection of minimal residual disease (MRD) and recurrence.
Transplant Monitoring: In organ transplant recipients, the quantification of donor-derived cfDNA serves as a highly sensitive, non-invasive biomarker for assessing graft rejection.
Research and Emerging Diagnostics: cfDNA analysis is being actively explored in other areas, including the study of autoimmune diseases and sepsis.

As a cornerstone of liquid biopsy, cfDNA analysis provides a powerful tool for precision medicine, offering critical insights that were previously inaccessible without invasive procedures.

Our Rebeads CfDNA Special D Cell Free DNA Extraction Magnetic Beads are engineered for the highly efficient and reliable isolation of short-fragment, low-concentration cfDNA from plasma, serum, and other liquid biopsies. Utilizing a specialized surface chemistry, these beads demonstrate superior binding affinity for dsDNA and ssDNA across a wide size range, ensuring maximum recovery of the critical 160–180 bp fraction that is rich in biological information. This technology is the cornerstone for robust and reproducible liquid biopsy workflows, enabling downstream applications in non-invasive prenatal testing (NIPT), cancer early detection, and transplant monitoring.

Specifications

Art. No.	NBF208
Appearance	Dark Brown To Black Liquid, Slight Odor.
Grain Size	300 nm
Concentration	50 mg/mL
Magnetic Reaction Time	< 30 s
Sediment Time	> 5 min
Application	Serum/plasma Free DNA/RNA
Storage Condition	2-8 ℃, Protected From Light. Do Not Freeze.
Shelf Life	24 months
Package	50 / 100 / 500 / 1000 mL
Free Sample	Available
Highlight	Special D Cf-DNA Extraction, Cell Free DNA Purification Exceptional Purity, Exceptional Purity Cf-DNA Extraction

Series Selection Guide

Parameter	cfDNA Special A	cfDNA Special B	cfDNA Special C	cfDNA Special D
Catalog No.	NBF205	NBF206	NBF207	NBF208
Particle Size	300 nm	300 nm	300 nm	300 nm
Concentration	50 mg/mL	50 mg/mL	100 mg/mL	50 mg/mL
Storage Buffer	Ultra pure water
Shelf Life	48 months from date of manufacture
Matrix	Silica magnetic beads
Applications	Cell-free DNA extraction	Cell-free DNA extraction	Cell-free DNA extraction	Cell-free DNA extraction
Suitable For	Serum/plasma cell-free DNA	Serum/plasma cell-free DNA	Serum/plasma cell-free DNA	Serum/plasma cell-free DNA
Remarks	For research use only

Features

Optimized for Short Fragments: Superior recovery efficiency for short, fragmented cfDNA, minimizing the loss of critical biomarkers.
High Sensitivity & Yield: Designed for maximum yield from low-input and low-concentration samples, which is essential for liquid biopsy applications.
Exceptional Purity: Effectively removes contaminants such as proteins, salts, and cellular genomic DNA, providing clean cfDNA ideal for sensitive downstream assays like NGS and dPCR.
Robust & Automation-Ready: Exhibits excellent chemical stability and dispersion, ensuring batch-to-batch consistency and seamless integration into automated, high-throughput nucleic acid extraction systems.
Rapid Workflow: Streamlines the extraction process, reducing hands-on time and enabling quick turnaround from sample to analysis.

This product is an indispensable tool for clinical researchers and diagnostic developers who require the highest standards of performance in their cfDNA analysis pipeline.

Extraction Procedure

Magnetic Beads Selection Guide

Precautions

Prevent Agglomeration: Avoid freezing and drying.
Ensure Proper Resuspension: Vortex before use.
Color Variation is Normal.

Documents

User Manual (PDF) MSDS (PDF) COA

Rebeads CfDNA Special D Cell-Free DNA Extraction Magnetic Beads