Description

Rat Resident Peritoneal Macrophages are isolated from the peritoneal cavity of 8-10 weeks old Sprague–Dawley Rats. Cells at passage 0 are cryo-preserved in vials. Each vial contains 1x10⁶ cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma and tested for expression of markers using antibody, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics. Primary Resident peritoneal macrophages can not be cultured indefinitely and can not be passaged.

Suggested Medium

Macrophage Medium /w Kit (500 ml)

Catalog No. M3368

Laboratory Applications

Rat Resident Peritoneal Macrophages can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.

Storage

Cell Biologics will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.

Documents

Cell-Culture-Protocol-CB

Disclaimer

Investigators should handle the cells that they receive from Cell Biologics with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Rat Resident Peritoneal Macrophages (Frozen Cells)