{"product_id":"rat-resident-peritoneal-macrophages-frozen-cells","title":"Rat Resident Peritoneal Macrophages (Frozen Cells)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRat Resident Peritoneal Macrophages are isolated from the peritoneal cavity of 8-10 weeks old Sprague–Dawley Rats. Cells at passage 0 are cryo-preserved in vials. Each vial contains 1x10\u003csup\u003e6\u003c\/sup\u003e cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma and tested for expression of markers using antibody, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003ePrimary Resident peritoneal macrophages can not be cultured indefinitely and can not be passaged.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003e  Macrophage Medium \/w Kit (500 ml)\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eCatalog No. M3368\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003eRat Resident Peritoneal Macrophages can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, ROS, or cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e will ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/RA-6032RF_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all Cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial 1 x 10^6","offer_id":52481272381621,"sku":"RA-6032RF","price":480.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/macrophage_copy-500x500_9ad40811-13f5-4a20-b84d-ba8028c1ec42.jpg?v=1778249460","url":"https:\/\/biofargo.com\/products\/rat-resident-peritoneal-macrophages-frozen-cells","provider":"Biofargo","version":"1.0","type":"link"}