Description

Rat Primary Hepatocytes from Cell Biologics are isolated from Sprague-Dawley rat liver tissues. Cells at passage 0 are cryo-preserved and each vial contains 3x10⁶ cells per ml. Rat Primary Hepatocytes are characterized by immunofluorescence staining with an antibody of ZO-1. Cells can be plated on culture plates for desired experiments under the cell culture conditions specified by Cell Biologics. Primary hepatocytes can not be cultured indefinitely and can not be passaged.

Suggested Medium

M1265 Complete Hepatocyte Medium /w Kit (500 ml)

Storage

Cryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-150°C) immediately until ready for use. Cells should be plated and used directly for desired studies within 1-2 weeks upon receipt. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

RA-6224

Cell-Culture-Protocol-CB

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Rat Primary Hepatocytes - Plateable