{"product_id":"rat-primary-cardiomyocyte","title":"Rat Primary cardiomyocyte","description":"\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eCardiomyocytes medium\/w Kit -125 ml\u003c\/p\u003e\n\u003cp\u003eCatalog No. M6368\u003c\/p\u003e\n\u003cp\u003eRat Primary Cardiomyocytes from \u003ci\u003eCell Biologics are derived from the heart of pathogen-free laboratory \u003c\/i\u003epost neonatal mice (day 1-3).  Cells\u003ci\u003e \u003c\/i\u003eare freshly isolated and shipped at passage 1. Cells are grown in T25 tissue culture flasks, 6-well plates or 12-well plates, which were pre-coated with gelatin-based solution and fibronectin and incubated in \u003ci\u003eCell Biologics’ \u003c\/i\u003eCulture Complete Growth Medium for 3-7 days. The beating cardiomyocytes were shipped and ready for customers’ experiments. The method we use to isolate primary cardiomyocytes cells was developed based on a combination of established and our proprietary methods. These cells are purified by removing fibroblasts and endothelial cells. These cells will not proliferate in culture and cannot be passaged.\u003c\/p\u003e\n\u003cp\u003eRat Primary Cardiomyocytes from \u003ci\u003eCell Biologics \u003c\/i\u003edisplay typical elongated and flattened morphology under light microscopy. Cells are tested for expression of cardiomyocyte marker using antibody cardiac Troponin I (cTnI, Catalog No. ab47003, from Abcam) or Myosin heavy chain (Catalog No. MA5-35613, from Thermofisher. Scientific) by immunofluorescence staining. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased.\u003c\/p\u003e\n\u003cp\u003eRat Primary Cardiomyocytes can be used for standard biochemical procedures including RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics \u003c\/i\u003eships with fresh complete culture medium. On receipt, cells are ready for experimental use. Cells can be maintained up to 7 days under the cell culture conditions specified by \u003ci\u003eCell Biologics\u003c\/i\u003e. Subculturing, freezing and thawing of cardiomyocytes are not recommended.  \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/RN-6368_RN-6368.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eRN-6368\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/RN-6368_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Live cells 2well (6-well)","offer_id":52481604583605,"sku":"RN-6368","price":860.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/logo-500x500_5136b0b0-eefa-4f40-836c-3cbf3bcafd27.jpg?v=1778252331","url":"https:\/\/biofargo.com\/products\/rat-primary-cardiomyocyte","provider":"Biofargo","version":"1.0","type":"link"}