{"product_id":"rat-pancreatic-islets","title":"Rat Pancreatic Islets","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRat Pancreatic Islets from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from the pancreas of Sprague–Dawley rat using Collagenase P and purified using Ficoll density gradient. The isolated islets are cultured in \u003ci\u003eCell Biologics’\u003c\/i\u003e Pancreatic Islet Culture Medium containing 5% FBS.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eRat Pancreatic Islets are tested for their viability using FDA\/PI, purity using Dithizone staining, static insulin secretion in response to 14 mM glucose. Upon request, dynamic insulin secretion and calcium imaging in response to glucose and other stimulators can be performed.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003ePancreatic islet culture medium \/w Kit – 100 ML\u003c\/p\u003e\n\u003cp\u003eCatalog No. \u003cb\u003eM2269\u003c\/b\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003eRat Pancreatic Islets can be used for the assay of islet function, drug testing, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or others for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships fresh islet cells on at either room temperature or 4°C. On receipt, immediately transfer the cells to 37°C and 5% CO\u003csub\u003e2\u003c\/sub\u003e incubator.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/RA-6232S_RA-6232S.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eRA-6232S\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/RA-6232S_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eAppropriate safety procedures should always be used with this material. Investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"300 islets Live","offer_id":52481507885237,"sku":"RA-6232S","price":860.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG_3477a-500x500_6cb5bda4-9d0e-4b4b-963b-9a03a39c4661.jpg?v=1778252249","url":"https:\/\/biofargo.com\/products\/rat-pancreatic-islets","provider":"Biofargo","version":"1.0","type":"link"}