{"product_id":"rat-lung-mesenchymal-stem-cells","title":"Rat Lung Mesenchymal Stem Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRat Lung Primary Mesenchymal Stem Cells are derived from the lung tissues of \u003c\/strong\u003eday-1 Sprague-Dawley Rats.\u003cstrong\u003e Rat Lung Primary Mesenchymal Stem Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Cell Biologics’ Cell Culture Medium for 7-15 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Rat Lung Primary Mesenchymal Stem Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded 3-6 passages by the ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics.\u003c\/i\u003e Repeated freezing and thawing of cells is not recommended. Mouse Lung Mesenchymal Stem Cells are tested for expression of markers using antibodies, CD29 and CD44 positive by flow cytometry.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003e  M5566 – Mesenchymal Stem Cell Medium \/w Kit - 500 ml\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRat Lung Primary Mesenchymal Stem Cells\u003c\/strong\u003e can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, or immunofluorescent flow cytometry. \u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request.\u003c\/p\u003e\n\u003cp\u003eNever can primary cells be kept at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/R2303_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eGeneral Protocol\u003c\/h2\u003e\n\u003cp\u003eAll cell culture procedures must be conducted in a bio-safety cabinet.\u003c\/p\u003e\n\u003cp\u003eAny and all media, supplements, and reagents must be sterilized by filtration through a 0.2 µm filter.\u003c\/p\u003e\n\u003cp\u003eUse aseptic technique to prevent microbial contamination.\u003c\/p\u003e\n\u003cp\u003eCryo-preserved cells must be stored in liquid nitrogen or seeded immediately upon arrival.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eMedium:\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eReview the information provided on the \u003ci\u003eCell Biologics’\u003c\/i\u003e website about appropriate culture media (e.g. serum and other supplements). Use pre-warmed (37°C) cell culture media (\u003cb\u003e30-50 ML\u003c\/b\u003e) to recover cryo-preserved cells and when changing media or splitting cells.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eCoating of flasks or dishes:\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eCoat sterile culture dishes or flasks with Gelatin-Based Coating Solution (\u003ci\u003eCell Biologics,\u003c\/i\u003e Catalog No. 6950) for 10-30 min and then aspirate the excess solution before seeding cells.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eCell recovery from cryovial:\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e•        Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for \u0026lt;1 min until there is just a small bit of ice left in the vial.\u003c\/p\u003e\n\u003cp\u003e•        Promptly remove the vial and wipe it down with 70% ethanol.\u003c\/p\u003e\n\u003cp\u003e•        Transfer cells from the vial to a sterile centrifuge tube. Add 8-10 ml of pre-warmed Cell Biologics Cell Culture Medium.\u003c\/p\u003e\n\u003cp\u003e•        Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.\u003c\/p\u003e\n\u003cp\u003e•        Centrifuge cells at 200 g for 5 minutes.\u003c\/p\u003e\n\u003cp\u003e•        Aspirate the supernatant and resuspend the cell pellet in 6 ml of \u003ci\u003eCell Biologics’\u003c\/i\u003e Cell Culture Growth Medium.\u003c\/p\u003e\n\u003cp\u003e•        \u003cb\u003eAdd resuspended cells into a T75 flask pre-coated with Gelatin-Based Coating Solution (\u003ci\u003eCell Biologics\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eAlthough \u003cstrong\u003eRat Lung Primary Mesenchymal Stem Cells\u003c\/strong\u003e are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from \u003cem\u003eCell Biologics\u003c\/em\u003e\u003cstrong\u003e \u003c\/strong\u003ewith caution, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":52481283424437,"sku":"R2303","price":560.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/WT_CFTR_CD11C_5-20-2008_red_copy-500x500.jpg?v=1778251539","url":"https:\/\/biofargo.com\/products\/rat-lung-mesenchymal-stem-cells","provider":"Biofargo","version":"1.0","type":"link"}