Description

Rat Bone Marrow Mesenchymal Stem Cells from Cell Biologics are isolated from Sprague-Dawley rat tibias and femurs and grown in tissue culture flasks with Cell Biologics’ Complete Growth Medium. Cells at passage 1-2 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml. Rat Bone Marrow Mesenchymal Stem Cells are characterized by immunofluorescence staining with antibodies of CD44, Sca-1 or CD29. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Suggested Medium

M5556 Mesenchymal Cell Medium w/ Kit (500 ml)

Storage

Cryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

RA-6043

Cell-Culture-Protocol-CB

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Publication

Stem Cells Transl Med. 2022 Oct; 11(10): 1089–1102.

Rat Bone Marrow Mesenchymal Stem Cells (RAMSC)