Description

Rat Astrocytes from Cell Biologics are isolated from brain tissues of Sprague-Dawley rat. Cells are grown in tissue culture flasks and incubated in Cell Biologics’ Cell Culture Medium for 14-21 days. Prior to shipping, cells at passage 1 are detached from flasks and cryopreserved in vials. Each vial contains 0.5x10⁶ cells per ml.

Product Testing

Rat Astrocytes from Cell Biologics are tested for expression of GFAP antibody by Immunofluorescence Staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

Suggested Medium

Complete Astrocyte Medium/w Kit – 500 ml

Catalog No. M2266

Laboratory Applications

Rat Astrocytes can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.

Storage

Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.

Documents

Cell-Culture-Protocol-CB

Disclaimer

Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.