{"product_id":"rapid-protein-staining-solution","title":"Rapid Protein Staining Solution","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRapid Protein Staining Solution is an advanced Coomassie Brilliant Blue G-250–based staining reagent designed for fast, sensitive, and convenient protein visualization.\u003c\/p\u003e\n\u003cp\u003eIt is suitable for staining protein gels following SDS-PAGE or native PAGE, as well as for detecting residual proteins in gels after Western blot transfer.\u003c\/p\u003e\n\u003cp\u003eProtein bands can be visualized within 2 minutes, with detection sensitivity reaching 100 ng within 5 minutes. With appropriate destaining, the detection limit can be as low as 10 ng.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRapid and Highly Sensitive\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eVisible protein bands appear within 2 minutes. Detection sensitivity reaches 100 ng in 5 minutes and can be improved to 10 ng with extended staining and proper destaining.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSimple and Time-Saving\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eNo fixation, heating, or pre-washing steps are required. Protein bands can be directly visualized after water destaining.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEnvironmentally Friendly\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFormulated with a non-toxic and non-hazardous composition, free of irritating odors, ensuring a safer working environment.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eSDS-PAGE protein gel staining\u003c\/p\u003e\n\u003cp\u003eNative PAGE protein gel staining\u003c\/p\u003e\n\u003cp\u003eDetection of residual proteins after Western blot transfer\u003c\/p\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eGel Preparation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAfter electrophoresis, briefly rinse the gel in deionized water to remove residual buffer and reduce background.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStaining\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eDiscard the rinse solution and add sufficient staining solution to fully immerse the gel.\u003c\/p\u003e\n\u003cp\u003eIncubate at room temperature on a horizontal shaker for 5–30 minutes.\u003c\/p\u003e\n\u003cp\u003eFor low-abundance proteins, longer staining times are recommended. Staining duration may be adjusted based on band intensity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eDestaining and Visualization\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eRemove the staining solution and rinse the gel with deionized water to eliminate excess dye. Protein bands will become visible immediately.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptional Background Reduction\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eReplace deionized water every 5 minutes and repeat 3–5 times to further reduce background.\u003c\/p\u003e\n\u003cp\u003eAlternatively, soak the gel in deionized water overnight for optimal background clarity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage and Stability\u003c\/h2\u003e\n\u003cp\u003eStore at room temperature, protected from light.\u003cbr\u003eStable for 12 months under recommended storage conditions.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cp\u003eThe recommended protocol is optimized for gels of 8 × 10 cm or 10 × 10 cm with a thickness of 0.75 mm. For larger or thicker gels, increase staining volume and extend incubation time accordingly.\u003c\/p\u003e\n\u003cp\u003eThe staining solution may be reused 5–6 times with a slight decrease in sensitivity. For reuse, store at 4°C and extend staining time as needed.\u003c\/p\u003e\n\u003cp\u003eUse at least 20 mL of staining solution per gel. Insufficient volume may compromise staining performance.\u003c\/p\u003e\n\u003cp\u003eMinor precipitates may form during storage. Mix thoroughly before use. This does not affect product performance.\u003c\/p\u003e\n\u003cp\u003eFor Research Use Only. Not for use in diagnostic or therapeutic procedures.\u003cbr\u003eAppropriate personal protective equipment (lab coat, gloves) should be worn during use.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eData Sheet\u003c\/strong\u003e\u003c\/p\u003e","brand":"Epoto Biotech","offers":[{"title":"500mL","offer_id":52392840069301,"sku":"BF1001-500ml","price":0.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/biofargo.com\/products\/rapid-protein-staining-solution","provider":"Biofargo","version":"1.0","type":"link"}