{"product_id":"quantaccuracy-rt-ramda-cdna-synthesis-kit","title":"QuantAccuracy RT-RamDA cDNA Synthesis Kit-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eThe QuantAccuracy™, RT-RamDA™ cDNA Synthesis Kit (Code No. RMQ-101) is an efficient and convenient kit to synthesize cDNA from single cells or trace amounts of RNA for real-time PCR analysis.\u003c\/p\u003e\n\u003cp style=\"margin-top: 0;\"\u003eBy using this kit, cDNA covering full-length total RNA can be synthesized, and gene expression analysis can be performed with high sensitivity.\u003c\/p\u003e\n\u003cp style=\"margin-top: 0;\"\u003eThis kit uses reverse transcription with random displacement amplification (RT-RamDA™) (see method in reference (1)). RT-RamDA™ is a novel cDNA amplification method that utilizes the strand displacement activity of reverse transcriptase, which can detect not only poly(A) RNA but also non-poly(A) RNA with high sensitivity. For this reason, the RT-RamDA™ method is characterized by its ability to detect more genes than conventional technology.\u003c\/p\u003e\n\u003cp style=\"margin-top: 0;\"\u003e*RT-RamDA™ and RamDA-seq™are trademarks of RIKEN, Institute of Physical and Chemical Research, Japan\u003cbr\u003eReference (1) Tetsutaro Hayashi*, Haruka Ozaki*, Yohei Sasagawa, Mana Umeda, Hiroki Danno and Itoshi Nikaido. Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs. Nature Communications. 2018.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e cDNA can be prepared from a single cell or a small amounts of input RNA\u003cbr\u003eBetween 1–500 cells or 10 pg–10 ng total RNA should be used.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Low-copy genes can be analyzed\u003cbr\u003eBy amplifying cDNA to tens of times from RNA, low-copy genes can be analyzed from samples containing small amounts of RNA.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Very accurate quantification\u003cbr\u003eAmplification occurs randomly in RT-RamDA™, which can reduce amplification bias.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Minimize input samples\u003cbr\u003eBecause amplified cDNA can be obtained, valuable samples are saved.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Can be used with a variety of real-time PCR reagents\u003cbr\u003eThis kit can be used in combination with a variety of real-time PCR reagents and is compatible with both SYBR® Green I and TaqMan® assays.\u003cbr\u003eTHUNDERBIRD™ qPCR Mix (Code No. QPS-101, QPS-201, QPX-201), and KOD SYBR® qPCR Mix (Code No. QKD-201) can be used.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_cdna-005_01.png?v=1775548483\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003cp\u003eThis is the example of preparing cDNA for purified RNA 10pg derived from HeLa cells using non-amplified reverse transcriptase or this product, and amplifying ACTB by real-time PCR.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eStorage condition\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eStore at -20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eComponents\u003c\/h2\u003e\n\u003cp\u003eThe kits include the following reagents that can be used for 96 reactions (RMQ-101) or 24 reactions(RMQ-101T). All reagents should be stored at -20℃.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 900px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eQuantAccuracy™, RT-RamDA™ cDNA Synthesis Kit\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eRMQ-101\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eRMQ-101T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e96 Reactions\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e24 Reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eLysis Buffer\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e480 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eLysis Enhancer\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e108 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e27 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRNase Inhibitor\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e22 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e6 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRT-RamDA™ Buffer\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e240 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003egDNA Remover\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e54 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e14 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRT-RamDA™ Enzyme Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e60 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e15 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRT-RamDA™ Primer Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e60 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e15 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eNuclease-free water\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e960 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e240 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e* Do not store mixed solution.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003ePrinciple of rt-ramda™ method\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eRT-RamDA™ is an abbreviation for \"Reverse Transcription with Random Displacement Amplification\" developed by the Bioinformatics Research and Development Team, RIKEN Center for Biosystems Science and Technology.It is a new cDNA amplification method that applies the strand substitution activity of reverse transcriptase, and it is possible to prepare cDNA not only from poly (A) RNA but also from non-poly (A) -derived RNA for using raondom primer.\u003cbr\u003eIn addition, because cDNA is amplified at the same time as it is synthesized, RT-RamDA™ method does not require amplification adaptors and PCR amplification step, and it reduces the biases caused by PCR amplification.\u003cbr\u003e*Hayashi .T et al. Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs. Nature Communications. 9：619（2018)\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0 0 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_cdna-005_02.png?v=1775548555\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 1.Comparison of the amount of cDNA between the QuantAccuracy™ and conventional Reverse Transcription Kit\u003c\/h3\u003e\n\u003cp\u003ecDNA synthesis was performed using 1 ng of total RNA extracted from HeLa S3 cells to compare the QuantAccuracy™, RT-RamDA™ cDNA Synthesis Kit (Code No. RMQ-101) with a conventional reverse transcription kit from Company A.\u003cbr\u003eReal-time PCR analysis was then performed on two types of long non-coding RNA genes (NEAT1 and MALAT1) and eight types of housekeeping genes.\u003cbr\u003eAs a result, a correlation of Ct value was observed between this product and the reverse transcription reagent without amplification ability, and ΔCt\u0026gt; 4.5 for all genes, and it was confirmed that this product has an amplification factor of 24.5≒20 times or more.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_cdna-005_03.png?v=1775548612\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003cp\u003eΔCt values between QuantAccuracy™ and a conventional Reverse Transcription Kit\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_cdna-005_04.png?v=1775548613\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003cp\u003eCorrelation between QuantAccuracy™ (y-axis) and a conventional Reverse Transcription Kit (x-axis, Company A).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 2.Analysis using a human FFPE tissue\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eMethod\u003c\/strong\u003e\u003cbr\u003ecDNA synthesis was performed using 100 pg or 1 ng RNA extracted from human FFPE tissue to compare the QuantAccuracy™, RT-RamDA™ cDNA Synthesis Kit (Code No.RMQ-101) with other cDNA amplification kits from competitors B and C, and a conventional reverse transcription kit from competitor D. As in Example 1, real-time PCR analysis was performed on two types of long non-coding RNA genes and seven types of housekeeping genes using THUNDERBIRD™ SYBR® qPCR Mix (Code No. QPS-201) with each cDNA used as a template.\u003cbr\u003eThe amount of cDNA in the QuantAccuracy™, RT-RamDA™ cDNA Synthesis Kit was calculated from the Ct value, and the value obtained by dividing by the amount of cDNA in the conventional reverse transcription reaction was calculated as the amplification rate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults\u003c\/strong\u003e\u003cbr\u003eThe detection rates of each gene from 100 pg RNA were compared. As a result, cDNA prepared using this product showed the highest detection rate for any of the genes.cDNA was prepared using different RNA amounts (100 pg, 1 ng) as a template, amplified by real-time PCR, and the Ct values were compared.As shown in the figure below, the Ct value when using 100 pg RNA and the Ct value when using 1 ng RNA are connected by a straight line.\u003cbr\u003eThere was almost no difference in amplification rate between each gene only when this product was used.In addition, the average ΔCt value between 100pg and 1ng of this product was about 3.40.In other words, in the analysis using this product, the difference in the amount of RNA 10 times can be evaluated as the difference in the amounts of gene 23.4≒ 10.6 times.For RNA derived from FFPE specimens, this product can be analyzed with little bias in amplification factor.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\n\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_cdna-005_09.png?v=1775548767\" alt=\"\" style=\"max-width: 100%; height: auto; margin-bottom: 10px;\"\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_cdna-005_05.png?v=1775548767\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 3.Preparation of cDNA from a single cell\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eMethod\u003c\/strong\u003e\u003cbr\u003eThrough Phorbol 12-myristate 13-acetate (PMA) treatment, a single-cell expression analysis experiment of the inflammatory cytokine gene IL-8 was performed. HeLa S3 cells were treated with 100 nM PMA for 24 hours, and then the cells were separated one by one using a cell sorter. cDNA was prepared from one separated cell (n = 48) using this product, and the IL-8 (CXCL8) gene was detected using THUNDERBIRD™ SYBR® qPCR Mix (Code No. QPS-201).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults\u003c\/strong\u003e\u003cbr\u003eIt was found that the Ct value was reduced by about 3 by PMA treatment and the expression level of IL-8 was significantly increased (p value was calculated from Student's t-test).By using this product, it was possible to analyze gene expression even in a single cell level.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_cdna-005_06.png?v=1775548791\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 4.Preparation of cDNA from trace RNA\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eMethod\u003c\/strong\u003e\u003cbr\u003eGenes that are thought to be the causative genes of chronic leukemia were investigated.10 pg of K562 cell-derived RNA (BCR-ABL (+)) was spiked into 1 ng, 5 ng, and 10 ng HeLa cell-derived RNA (BCR-ABL (-)). Then, cDNA was prepared using each reverse transcription reagent, and the BCR-ABL gene was detected by real-time PCR using THUNDERBIRD™ SYBR® qPCR Mix (Code No. QPS-201) (n = 4).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults\u003c\/strong\u003e\u003cbr\u003eAs the amount of RNA derived from HeLa cells increased, the efficiency of cDNA synthesis from trace of RNA decreased with the reverse transcription reagent without amplification ability, and detection omission occurred. Our products were able to efficiently prepare cDNA and all were detectable.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_cdna-005_07.png?v=1775548824\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 5.Input amount and number of detected genes\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eMethod\u003c\/strong\u003e\u003cbr\u003ecDNA was prepared from iPS cell-derived RNA (1 ng, 100 ng) using each reverse transcription reagent. Then, it was added to TaqMan Array Mouse Stem Cell Pluripotency, and 96 kinds of genes were detected by THUNDERBIRD™ Probe qPCR Mix (Code No. QPS-101).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults\u003c\/strong\u003e\u003cbr\u003eIn the input amount of RNA1ng, this product was able to detect more genes than the reagent of Company C. In addition, almost the same number of genes were detected as when cDNA was prepared from 100 times the amount of RNA (100 ng) using the company C reverse transcription reagent.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_cdna-005_08.png?v=1775548824\" alt=\"\" style=\"max-width: 100%; height: auto;\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\n  \u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RMQ-101_manual_EN.pdf?v=1775807409\" target=\"_blank\"\u003e Download Manual \u003c\/a\u003e \n\n  \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RMQ-101_EN_SDS.pdf?v=1775807402\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e\n\n\u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RMQ-101_Eng.pdf?v=1775807411\" target=\"\\_blank\"\u003e Download Flyer \u003c\/a\u003e\n\n\u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Toyobo","offers":[{"title":"96Rxn","offer_id":52417810596021,"sku":"RMQ-101","price":636.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/QuantAccuracy_RT-RamDA_cDNA_Synthesis_Kit-Toyobo.jpg?v=1777517346","url":"https:\/\/biofargo.com\/products\/quantaccuracy-rt-ramda-cdna-synthesis-kit","provider":"Biofargo","version":"1.0","type":"link"}