Specifications

Grade	Biotech
CAS	39450-01-6
Appearance	White lyophilized powder
Activity(hemoglobin:ph7.5;37℃)	≥30.0 mAnsonU/mg protein
DNase(Nlcking activity;Pbr 322; 37℃)	None detectable
RNase activity	None detectable
Exonucleases	None detectable
Storage	-20ºC. Protect from moisture. Warm to room temperature before opening

Application

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Biochem/physiol Actions

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Documents

COA

SDS

Disclaimer: For laboratory research use only.