{"product_id":"probe-qpcr-master-mix-universal-starlighter","title":"Probe qPCR Master Mix (Universal)——StarLighter","description":"\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\n\u003cp\u003eProbe-based qPCR is a demanding technique: non-specific amplification and poor reproducibility of results are common problems in the laboratory, especially when target templates have a high GC content or are present at low copy numbers.\u003c\/p\u003e\n\n\u003cp\u003eStarLighter Probe qPCR Master Mix (Universal) is a universal probe qPCR master mix based on a \u003cstrong\u003ehot-start engineered Taq DNA polymerase\u003c\/strong\u003e and an optimized buffer. It effectively \u003cstrong\u003ereduces non-specific amplification\u003c\/strong\u003e and significantly improves the efficiency of probe-based real-time PCR. With its high sensitivity and broad quantitative range, it enables accurate quantitative and qualitative analysis of target genes as well as SNP detection.\u003c\/p\u003e\n\n\u003cp\u003eFor laboratories performing gene expression analysis or SNP genotyping, this universal probe qPCR master mix offers excellent reproducibility and high reliability, and is compatible with a variety of qPCR probes.\u003c\/p\u003e\n\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\n\u003cdiv style=\"overflow-x:auto;-webkit-overflow-scrolling:touch;\"\u003e\n  \u003ctable style=\"border-collapse:collapse;width:100%;min-width:560px;\"\u003e\n    \u003cthead\u003e\n      \u003ctr\u003e\n        \u003cth style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;font-weight:600;white-space:nowrap;\"\u003eCatalog No.\u003c\/th\u003e\n        \u003cth style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;font-weight:600;white-space:nowrap;\"\u003eSize\u003c\/th\u003e\n        \u003cth style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;font-weight:600;white-space:nowrap;\"\u003ePackaging\u003c\/th\u003e\n      \u003c\/tr\u003e\n    \u003c\/thead\u003e\n    \u003ctbody\u003e\n      \u003ctr\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003eFS-Q2002\u003c\/td\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003e5 ml\u003c\/td\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003e4 × 1.25 ml per box\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \u003ctr\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003eFS-Q2001-S\u003c\/td\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003e1 ml\u003c\/td\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003e1 tube per box\u003c\/td\u003e\n      \u003c\/tr\u003e\n    \u003c\/tbody\u003e\n  \u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eStrong amplification specificity\u003c\/strong\u003e — reliable data results.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eHigh GC template amplification\u003c\/strong\u003e — amplifies complex templates that are difficult to amplify with ordinary master mixes.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eHigh amplification efficiency and earlier Ct value\u003c\/strong\u003e — target is detected earlier in the real-time PCR run.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eHigh signal-to-noise ratio and high sensitivity\u003c\/strong\u003e — suitable for low copy detection.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eHot-start engineered Taq DNA polymerase\u003c\/strong\u003e with optimized buffer — effectively reduces non-specific amplification.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBroad quantitative range\u003c\/strong\u003e — accurate quantitative and qualitative analysis of target genes, including SNP detection.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eExcellent reproducibility and high reliability\u003c\/strong\u003e — compatible with a variety of qPCR probes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\n\u003cp\u003eStarLighter Probe qPCR Master Mix (Universal) is a universal probe qPCR master mix suitable for:\u003c\/p\u003e\n\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eGene expression analysis\u003c\/strong\u003e — accurate quantification of target genes.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSNP and allele typing\u003c\/strong\u003e — SNP detection and genotyping assays.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eValidation of sequencing and microarray results\u003c\/strong\u003e — confirmatory real-time PCR.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat is StarLighter Probe qPCR Master Mix (Universal)?\u003c\/summary\u003e\n  \u003cp\u003eIt is a universal probe qPCR master mix based on a hot-start engineered Taq DNA polymerase and an optimized buffer, designed for high-sensitivity probe-based qPCR detection with a broad quantitative range.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eHow does this master mix reduce non-specific amplification?\u003c\/summary\u003e\n  \u003cp\u003eThe master mix is based on a hot-start engineered Taq DNA polymerase with an optimized buffer, which effectively reduces non-specific amplification and significantly improves qPCR efficiency.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhich qPCR probes are compatible with this master mix?\u003c\/summary\u003e\n  \u003cp\u003eThis universal probe qPCR master mix is compatible with a variety of qPCR probes.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eHow does it perform with high GC templates?\u003c\/summary\u003e\n  \u003cp\u003eIt provides strong high GC template amplification. In a test with the ApoE3 gene (197 bp, 71% GC) at template amounts of 50, 5, 0.5 and 0.05 ng, the master mix amplified the template effectively, while a supplier K product failed (Ct \u0026gt; 45).\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat is the amplification efficiency of this master mix?\u003c\/summary\u003e\n  \u003cp\u003eIn a test with the reference gene Actin (179 bp) using serial dilutions, the Ct value was reached approximately 1–1.5 cycles earlier, with better efficiency and R² compared with the reference.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat is the low copy sensitivity of this master mix?\u003c\/summary\u003e\n  \u003cp\u003eUsing a 5-fold serial dilution starting from 100 ng, the master mix amplified effectively down to 0.032 ng, with a good linear relationship of Ct values.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eHow stable is this master mix?\u003c\/summary\u003e\n  \u003cp\u003eIn stability tests, after 30 freeze-thaw cycles, 5 days at 37 °C, 28 days at room temperature and 43 days at 4 °C, no significant decrease in signal intensity or Ct value was observed (tested on a Thermo Q6 instrument).\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat applications is this master mix suitable for?\u003c\/summary\u003e\n  \u003cp\u003eIt is suitable for gene expression analysis, SNP and allele typing, and validation of sequencing and microarray results.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat are the available sizes?\u003c\/summary\u003e\n  \u003cp\u003eThe master mix is available as catalog No. FS-Q2002 (5 ml, packaged as 4 × 1.25 ml per box) and FS-Q2001-S (1 ml, packaged as 1 tube per box).\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eCan this master mix be used for SNP detection?\u003c\/summary\u003e\n  \u003cp\u003eYes. With its broad quantitative range and high sensitivity, it supports accurate quantitative and qualitative analysis of target genes as well as SNP detection.\u003c\/p\u003e\n\u003c\/details\u003e\n","brand":"Qihengxing","offers":[{"title":"1 ml (1 tube\/box)","offer_id":53196519506101,"sku":"FS-Q2001-S","price":0.0,"currency_code":"USD","in_stock":false},{"title":"5 ml (4 × 1.25 mL\/box)","offer_id":53196519538869,"sku":"FS-Q2002","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/fs-q2002-orange-logo-only-1500x1500_4d7da972-074f-4582-a07b-f481e6dbd998.jpg?v=1788317031","url":"https:\/\/biofargo.com\/products\/probe-qpcr-master-mix-universal-starlighter","provider":"Biofargo","version":"1.0","type":"link"}