{"product_id":"probe-based-multiplex-qpcr-master-mix-universal-starlighter","title":"Probe-Based Multiplex qPCR Master Mix (Universal)——StarLighter","description":"\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\n\u003cp\u003eMultiplex probe-based qPCR detection places high demands on the reagent: when several target sequences with very different GC contents are amplified in a single reaction, and the templates are derived from complex sample types, the performance of a conventional master mix often drops — some targets show weaker signals or lower sensitivity, and the reliability of the whole assay can be compromised.\u003c\/p\u003e\n\n\u003cp\u003eStarLighter Probe-Based Multiplex qPCR Master Mix (Universal) is a multiplex qPCR master mix developed specifically for \u003cstrong\u003emulti-target detection\u003c\/strong\u003e. The polymerase has been improved through \u003cstrong\u003eenzyme modification and directed evolution screening\u003c\/strong\u003e, combined with an optimized buffer reaction system, resulting in significantly enhanced \u003cstrong\u003einhibitor tolerance\u003c\/strong\u003e, broad sample compatibility and wide \u003cstrong\u003eGC compatibility\u003c\/strong\u003e. This probe-based multiplex qPCR master mix significantly improves the \u003cstrong\u003esensitivity\u003c\/strong\u003e, \u003cstrong\u003especificity\u003c\/strong\u003e and \u003cstrong\u003elow-copy detection\u003c\/strong\u003e of multiplex targets, while delivering high reproducibility, excellent amplification linearity and accurate quantification of target genes.\u003c\/p\u003e\n\n\u003cp\u003eFor laboratories running pathogen detection or disease detection panels, this universal multiplex qPCR master mix keeps multiple targets in one reaction performing consistently — across templates extracted from fecal samples, exfoliated cells, blood, serum\/plasma, plasmids and pathogens.\u003c\/p\u003e\n\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\n\u003cdiv style=\"overflow-x:auto;-webkit-overflow-scrolling:touch;\"\u003e\n  \u003ctable style=\"border-collapse:collapse;width:100%;min-width:560px;\"\u003e\n    \u003cthead\u003e\n      \u003ctr\u003e\n        \u003cth style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;font-weight:600;white-space:nowrap;\"\u003eCatalog No.\u003c\/th\u003e\n        \u003cth style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;font-weight:600;white-space:nowrap;\"\u003eSize\u003c\/th\u003e\n        \u003cth style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;font-weight:600;white-space:nowrap;\"\u003ePackaging\u003c\/th\u003e\n      \u003c\/tr\u003e\n    \u003c\/thead\u003e\n    \u003ctbody\u003e\n      \u003ctr\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003eFS-Q3011\u003c\/td\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003e5 ml\u003c\/td\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003e4 × 1.25 ml per box\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \u003ctr\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003eFS-Q3010\u003c\/td\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003e1 ml\u003c\/td\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003e1 tube per pack\u003c\/td\u003e\n      \u003c\/tr\u003e\n    \u003c\/tbody\u003e\n  \u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDeveloped specifically for multiplex qPCR\u003c\/strong\u003e — the polymerase is improved through enzyme modification and directed evolution screening with an optimized buffer reaction system, delivering high sensitivity, high reproducibility and stable performance for multi-target detection.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eWide GC compatibility\u003c\/strong\u003e — supports targets with a GC content ranging from 25% to 70%, allowing assays with different GC contents to run in the same reaction.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eEnhanced inhibitor tolerance and broad sample compatibility\u003c\/strong\u003e — suitable for templates extracted from fecal samples, exfoliated cells, blood, serum\/plasma, plasmids and pathogens, across multiple scenarios and applications.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eHigher fluorescence signal\u003c\/strong\u003e — produces stronger fluorescence signals and improved amplification curve shapes in multiplex reactions.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eExcellent multiplex performance on low template amounts\u003c\/strong\u003e — in multiplex amplification of four targets with different GC contents from 0.1 ng of gDNA, this master mix shows higher sensitivity than master mixes from other manufacturers.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eHigh specificity and low-copy detection\u003c\/strong\u003e — significantly improves the sensitivity, specificity and low-copy detection rate of multiplex targets.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSimple 2× premix format\u003c\/strong\u003e — just add primers, probes and template to run the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\n\u003cp\u003eStarLighter Probe-Based Multiplex qPCR Master Mix (Universal) is suitable for:\u003c\/p\u003e\n\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003ePathogen diagnosis\u003c\/strong\u003e — multiplex detection of pathogen-related targets in a single reaction.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDisease detection\u003c\/strong\u003e — simultaneous detection of multiple disease-related targets for efficient screening.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat is StarLighter Probe-Based Multiplex qPCR Master Mix (Universal)?\u003c\/summary\u003e\n  \u003cp\u003eIt is a probe-based multiplex qPCR master mix developed specifically for multi-target detection. The polymerase has been improved through enzyme modification and directed evolution screening, combined with an optimized buffer reaction system, significantly enhancing inhibitor tolerance, sample compatibility and GC compatibility for multiplex reactions.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eHow many targets can be detected in a single reaction?\u003c\/summary\u003e\n  \u003cp\u003eIn multiplex amplification of four targets with different GC contents using 0.1 ng of gDNA as the starting template, this master mix demonstrates strong fluorescence signals, good amplification curve linearity and high sensitivity.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat GC content range does this master mix support?\u003c\/summary\u003e\n  \u003cp\u003eIt supports targets with a GC content ranging from 25% to 70%, which makes it suitable for multiplex assays whose targets differ widely in GC content.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhich sample types is this multiplex master mix compatible with?\u003c\/summary\u003e\n  \u003cp\u003eIt is compatible with nucleic acid templates extracted from fecal samples, exfoliated cells, blood, serum\/plasma, plasmids and pathogens, covering multiple sample scenarios.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eHow does this master mix handle inhibitors in multiplex reactions?\u003c\/summary\u003e\n  \u003cp\u003eThe enzyme modification and directed evolution screening improve the polymerase configuration, and the optimized buffer reaction system enhances inhibitor tolerance, helping multiplex assays perform consistently with templates from complex sample sources.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhy does multiplex performance remain strong across different targets?\u003c\/summary\u003e\n  \u003cp\u003eThis probe-based multiplex qPCR master mix is optimized for multi-target detection: it produces higher fluorescence signals and better amplification curve shapes, and significantly improves the sensitivity, specificity and low-copy detection of multiplex targets with high reproducibility and excellent amplification linearity.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eIs this master mix suitable for single-target assays as well?\u003c\/summary\u003e\n  \u003cp\u003eThis master mix is designed for multiplex probe-based qPCR. For single-target probe assays, StarLighter also offers the Probe qPCR Master Mix (Universal) and the High Sensitivity Probe qPCR Master Mix (Universal) in the same product line.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat is the format of this 2× premix?\u003c\/summary\u003e\n  \u003cp\u003eIt is a 2× premix: just add primers, probes and template, then run the multiplex reaction.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat are the available sizes?\u003c\/summary\u003e\n  \u003cp\u003eThe master mix is available as catalog No. FS-Q3011 (5 ml, packaged as 4 × 1.25 ml per box) and FS-Q3010 (1 ml, packaged as 1 tube per pack).\u003c\/p\u003e\n\u003c\/details\u003e\n","brand":"Qihengxing","offers":[{"title":"1 ml (1 tube\/box)","offer_id":53197483475125,"sku":"FS-Q3010","price":0.0,"currency_code":"USD","in_stock":false},{"title":"5 ml (4 × 1.25 mL\/box)","offer_id":53197483507893,"sku":"FS-Q3011","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/exec-3468008c-4d84-47cf-aa90-4e641c4b4a7b.png?v=1788331843","url":"https:\/\/biofargo.com\/products\/probe-based-multiplex-qpcr-master-mix-universal-starlighter","provider":"Biofargo","version":"1.0","type":"link"}