Description

Precast-GLgel Hepes SDS-PAGE 4–20% (10-Well) is a ready-to-use gradient gel designed for high-resolution protein electrophoresis. Using glass plate construction, it minimizes non-specific adsorption and produces sharper, clearer protein bands. This 4–20% HEPES-based gradient gel is pre-cast and supplied with running buffer, eliminating the need for hand-casting and greatly improving reproducibility.

The wide gradient range and stable HEPES buffer system make the gel especially suitable for separating large molecular weight proteins and membrane protein complexes, which often require gentle handling and high-resolution gradient gels.

Specification

Full name	Precast-GLgel Hepes SDS-PAGE 4–20% 10-Well
Package	10 pcs
Components	10 pcs gel, 10 pcs SDS-PAGE running buffer
Gel size	8 × 7.4 × 0.15 cm
Plate size	9.8 × 8.4 × 0.41 cm
Wells	10 wells
Buffer system	HEPES buffer system
Maximum sample loading volume	60 µL
Recommended sample loading volume	30 µL

Features

Glass plate construction reduces non-specific protein adsorption, producing sharper and more defined protein bands.
Automated gel casting ensures batch stability and excellent reproducibility.
Wide 4–20% gradient provides strong resolving power across a broad molecular weight range.
Ideal for large molecular weight proteins — the gentle HEPES buffer system and gradient formulation help maintain separation quality of high-MW targets.
Optimized for membrane protein complexes — suitable for samples requiring mild conditions to preserve complex stability during electrophoresis.
SDS-free gel matrix allows both denaturing (SDS-PAGE) and non-denaturing (Native-PAGE) applications.
Ready-to-use format eliminates gel preparation time and reduces potential preparation errors.
Long shelf life and convenient storage at 2–8°C.

Applications

SDS-PAGE for general protein separation
Separation of large molecular weight proteins
Analysis of membrane protein complexes and other fragile protein assemblies
Native-PAGE for non-denaturing analysis
Western blot (WB) preparation and protein detection workflows
Any electrophoresis experiment requiring a 4–20% gradient gel

Storage

2~8°C, do not freeze

Precast-Gel Hepes SDS-PAGE 4-20% 10Well