Description

Precast-GLgel Hepes SDS-PAGE 4–15% (10-Well) is a ready-to-use gradient gel designed for efficient and high-resolution protein electrophoresis. Using glass plate construction, it minimizes non-specific adsorption and produces sharper, clearer protein bands.
This 4–15% HEPES-based gradient gel offers excellent separation performance for medium to large molecular weight proteins, and provides gentler running conditions suitable for membrane proteins and protein complexes. Pre-cast and supplied with running buffer, it eliminates the need for manual gel preparation, ensuring convenience, time savings, and better reproducibility across experiments.

Specification

Full name	Precast-GLgel Hepes SDS-PAGE 4–15% 10-Well
Package	10 pcs
Components	10 pcs gel, 10 pcs SDS-PAGE running buffer
Gel size	8 × 7.4 × 0.15 cm
Plate size	9.8 × 8.4 × 0.41 cm
Wells	10 wells
Buffer system	HEPES buffer system
Maximum sample loading volume	60 µL
Recommended sample loading volume	30 µL

Buffer

Buffer	Hepes Running Buffer for SDS-PAGE (Powder)
Specification	10*500 mL
Introduction	Hepes Running buffer is formulated for separation of proteins in their denaturing or nondenaturing state on Hepes-Tris gels. This product is available in powder form, a total of 10 packs, each pack can prepare 500 mL of 1× working solution.
Protocol (for reference only)	Take 1 pack of powder that can prepare 500 mL of hepes running buffer, pour it into a clean beaker, add 400 mL distilled water and stir to dissolve the powder completely. Make up to 500 mL in the graduated cylinder with distilled water, which is 1× working solution and can be used after mixing. Unused running buffer can be stored at room temperature, usually 2-3 days at room temperature and 1-2 weeks at 4°C.
Notes	1× working buffer should not be used for more than 2 weeks after preparation. This running buffer is suitable for Hepes-Tris gels, and should not be used for gels with other chemistries. For research use only. Not for use in diagnostic procedures. For your safety and health, please wear personal protective equipment and clothing when operating.
Storage	Store at room temperature, valid for 2 years.

Features

Glass plate design minimizes non-specific protein adsorption and produces sharper, clearer bands.
Automated gel casting ensures high consistency, stability, and reproducibility across batches.
4–15% gradient formulation provides excellent separation for medium to high molecular weight proteins.
Suitable for membrane proteins and protein complexes, offering gentle running conditions provided by the HEPES buffer system.
SDS-free gel matrix supports both denaturing (SDS-PAGE) and non-denaturing (Native-PAGE) applications.
Ready-to-use format eliminates manual gel preparation, saving time and reducing procedural variability.
Long shelf life and easy storage at 2–8°C.

Applications

SDS-PAGE analysis of general proteins
Separation of medium to large molecular weight proteins
Electrophoresis of membrane proteins and membrane protein complexes
Native-PAGE for non-denaturing protein studies
Western blot (WB) sample preparation and protein detection
Protein analysis workflows requiring a 4–15% gradient gel

Storage

2~8°C, do not freeze

Precast-Gel Hepes-Tris SDS-PAGE 4-15% 10Well