Description

Precast-GLgel Hepes SDS-PAGE 10% (10-Well) is a ready-to-use electrophoresis gel designed for effective separation of medium to high molecular weight proteins. Manufactured with an innovative glass plate design, it significantly reduces non-specific protein adsorption and provides clearer, sharper bands.

This 10% HEPES-based precast gel is supplied fully prepared along with compatible running buffer, allowing users to load samples directly without making gels manually. It improves workflow efficiency and delivers consistent performance across experiments.

The 10% gel concentration and mild HEPES buffer system also make it suitable for analyzing membrane proteins and membrane protein complexes, which benefit from gels with moderate pore size and stable, low-interference running conditions.

Specification

Full name	Precast-GLgel Hepes SDS-PAGE 10% 10-Well
Package	10 pcs
Components	10 pcs gel, 10 pcs SDS-PAGE running buffer
Gel size	8 × 7.4 × 0.15 cm
Plate size	9.8 × 8.4 × 0.41 cm
Wells	10 wells
Buffer system	HEPES buffer system
Maximum sample loading volume	60 µL
Recommended sample loading volume	30 µL

Buffer

Buffer	Hepes Running Buffer for SDS-PAGE (Powder)
Specification	10*500 mL
Introduction	Hepes Running buffer is formulated for separation of proteins in their denaturing or nondenaturing state on Hepes-Tris gels. This product is available in powder form, a total of 10 packs, each pack can prepare 500 mL of 1× working solution.
Protocol (for reference only)	Take 1 pack of powder that can prepare 500 mL of hepes running buffer, pour it into a clean beaker, add 400 mL distilled water and stir to dissolve the powder completely. Make up to 500 mL in the graduated cylinder with distilled water, which is 1× working solution and can be used after mixing. Unused running buffer can be stored at room temperature, usually 2-3 days at room temperature and 1-2 weeks at 4°C.
Notes	1× working buffer should not be used for more than 2 weeks after preparation. This running buffer is suitable for Hepes-Tris gels, and should not be used for gels with other chemistries. For research use only. Not for use in diagnostic procedures. For your safety and health, please wear personal protective equipment and clothing when operating.
Storage	Store at room temperature, valid for 2 years.

Features

Glass plate construction greatly reduces non-specific protein adsorption, resulting in sharper and clearer protein bands.
Automated gel filling technology ensures high stability and excellent reproducibility between batches.
10% gel concentration provides optimal resolution for medium to high molecular weight proteins.
Suitable for membrane proteins and membrane protein complexes, supported by the moderate pore size and stable HEPES buffer environment.
SDS-free gel matrix can be used for both denaturing electrophoresis (SDS-PAGE) and non-denaturing electrophoresis (Native-PAGE).
Precast and ready to use, eliminating the need for manual gel preparation and saving significant time.
Long shelf life and high compatibility, easily stored at 2–8°C.

Applications

SDS-PAGE protein separation
Analysis of medium to high molecular weight proteins
Electrophoresis of membrane proteins and membrane protein complexes
Native-PAGE (non-denaturing electrophoresis)
Western blot (WB) sample preparation and detection
Experiments requiring a 10% polyacrylamide gel

Storage

2~8°C, do not freeze

Precast-Gel Hepes-Tris SDS-PAGE 10% 10Well