Description

Precast-GLgel Hepes Native-PAGE 7.5% (10-Well) is a ready-to-use, non-denaturing gel designed for the analysis of proteins in their native, biologically active state. Constructed with an innovative glass plate design, it minimizes non-specific protein adsorption and delivers sharp, clear protein bands.

This 7.5% HEPES-based precast gel comes fully prepared with matched Native-PAGE running buffer, allowing direct sample loading without manual gel preparation. The 7.5% concentration is optimized for resolving larger molecular weight native proteins, including membrane proteins and native protein complexes, maintaining structural integrity while enabling gentle separation.

Specification

Full name	Precast-GLgel Hepes Native-PAGE 7.5% 10-Well
Package	10 pcs
Components	10 pcs gel, 10 pcs Native-PAGE running buffer
Gel size	8 × 7.4 × 0.15 cm
Plate size	9.8 × 8.4 × 0.41 cm
Wells	10 wells
Buffer system	HEPES buffer system
Maximum sample loading volume	60 µL
Recommended sample loading volume	30 µL

Features

Glass plate construction minimizes non-specific protein adsorption, producing sharper and clearer protein bands.
Automatic gel filling technology ensures consistent quality and excellent batch-to-batch reproducibility.
7.5% acrylamide concentration provides optimal resolution for larger molecular weight native proteins.
Suitable for membrane proteins and membrane protein complexes, preserving structural integrity under non-denaturing HEPES conditions.
Supports separation of native protein complexes, including oligomers and multi-subunit assemblies.
SDS-free gel matrix, ideal for true Native-PAGE studies and downstream functional analyses.
Ready-to-use precast format eliminates manual gel preparation, saving significant laboratory time.
Long shelf life with convenient storage at 2–8°C.

Applications

Native-PAGE analysis of proteins in non-denatured form
Separation of larger molecular weight native proteins
Electrophoresis of membrane proteins and membrane protein complexes
Analysis of native oligomers and protein complexes
Western blot (WB) after native separation
Experiments requiring a 7.5% native gel

Storage

2~8°C, do not freeze

Precast-Gel Hepes Native-PAGE 7.5% 10Well