Description

Precast-GLgel Hepes Native-PAGE 15% (10-Well) is a ready-to-use, non-denaturing gel designed for analyzing proteins in their native, biologically active conformations. The gel is constructed with an innovative glass plate design, which greatly reduces non-specific protein adsorption and ensures clearer and sharper native protein bands.

This 15% HEPES-based precast gel is supplied fully prepared with matched Native-PAGE running buffer, enabling direct sample loading without the need for manual gel formulation. The high acrylamide concentration offers excellent resolving power for small to medium molecular weight native proteins, and is also suitable for stabilizing compact membrane proteins and specific native protein complexes that benefit from a tighter gel matrix.

Specification

Full name	Precast-GLgel Hepes Native-PAGE 15% 10-Well
Package	10 pcs
Components	10 pcs gel, 10 pcs Native-PAGE running buffer
Gel size	8 × 7.4 × 0.15 cm
Plate size	9.8 × 8.4 × 0.41 cm
Wells	10 wells
Buffer system	HEPES buffer system
Maximum sample loading volume	60 µL
Recommended sample loading volume	30 µL

Features

Glass plate construction effectively reduces non-specific protein adsorption, producing clearer and sharper native protein bands.
Automatic gel filling technology ensures consistent quality and excellent batch-to-batch reproducibility.
High-concentration 15% native gel provides strong resolving capacity for small to medium molecular weight native proteins.
Suitable for membrane proteins, especially compact or stable membrane-associated proteins that require a firm gel matrix.
Supports analysis of specific native protein complexes where high-percentage gels enhance band tightness and resolution.
SDS-free gel, ideal for non-denaturing electrophoresis and preserving protein structure and biological activity.
Ready-to-use precast format significantly reduces preparation time and eliminates manual gel-making steps.
Long shelf life, easily stored at 2–8°C.

Applications

Native-PAGE analysis of proteins in non-denatured form
Separation of small to medium molecular weight native proteins
Electrophoresis of membrane proteins
Analysis of native protein complexes that require high-percentage gels
Western blot (WB) after native separation
Experiments requiring a 15% native gel for compact protein resolution

Storage

2~8°C, do not freeze

Precast-Gel Hepes Native-PAGE 15% 10Well