Description

Precast-GLgel Hepes Native-PAGE 15% (10-Well) is a ready-to-use, non-denaturing gel designed for analyzing proteins in their native, biologically active conformations. The gel is constructed with an innovative glass plate design, which greatly reduces non-specific protein adsorption and ensures clearer and sharper native protein bands.

This 15% HEPES-based precast gel is supplied fully prepared with matched Native-PAGE running buffer, enabling direct sample loading without the need for manual gel formulation. The high acrylamide concentration offers excellent resolving power for small to medium molecular weight native proteins, and is also suitable for stabilizing compact membrane proteins and specific native protein complexes that benefit from a tighter gel matrix.

Specification

Full name	Precast-GLgel Hepes Native-PAGE 15% 10-Well
Package	10 pcs
Components	10 pcs gel, 10 pcs Native-PAGE running buffer
Gel size	8 × 7.4 × 0.15 cm
Plate size	9.8 × 8.4 × 0.41 cm
Wells	10 wells
Buffer system	HEPES buffer system
Maximum sample loading volume	60 µL
Recommended sample loading volume	30 µL

Buffers

Buffer	Hepes Running Buffer for Native PAGE (Powder)
Specification	10*500 mL
Introduction	Hepes Running buffer is formulated for separation of proteins in their denaturing or nondenaturing state on Hepes-Tris gels. This product is available in powder form, a total of 10 packs, each pack can prepare 500 mL of 1× working solution.
Protocol (for reference only)	Take 1 pack of powder that can prepare 500 mL of hepes running buffer, pour it into a clean beaker, add 400 mL distilled water and stir to dissolve the powder completely. Make up to 500 mL in the graduated cylinder with distilled water, which is 1× working solution and can be used after mixing. Unused running buffer can be stored at room temperature, usually 2-3 days at room temperature and 1-2 weeks at 4°C.
Notes	1× working buffer should not be used for more than 2 weeks after preparation. This running buffer is suitable for Hepes-Tris gels, and should not be used for gels with other chemistries. For research use only. Not for use in diagnostic procedures. For your safety and health, please wear personal protective equipment and clothing when operating.
Storage	Store at room temperature, valid for 2 years.

Features

Glass plate construction effectively reduces non-specific protein adsorption, producing clearer and sharper native protein bands.
Automatic gel filling technology ensures consistent quality and excellent batch-to-batch reproducibility.
High-concentration 15% native gel provides strong resolving capacity for small to medium molecular weight native proteins.
Suitable for membrane proteins, especially compact or stable membrane-associated proteins that require a firm gel matrix.
Supports analysis of specific native protein complexes where high-percentage gels enhance band tightness and resolution.
SDS-free gel, ideal for non-denaturing electrophoresis and preserving protein structure and biological activity.
Ready-to-use precast format significantly reduces preparation time and eliminates manual gel-making steps.
Long shelf life, easily stored at 2–8°C.

Applications

Native-PAGE analysis of proteins in non-denatured form
Separation of small to medium molecular weight native proteins
Electrophoresis of membrane proteins
Analysis of native protein complexes that require high-percentage gels
Western blot (WB) after native separation
Experiments requiring a 15% native gel for compact protein resolution

Storage

2~8°C, do not freeze

Precast-Gel Hepes-Tris Native-PAGE 15% 10Well